Lung-resident CD4+ T cells are sufficient for IL-4Rα-dependent recall immunity to Nippostrongylus brasiliensis infection

• Published in: Mucosal immunology Vol. 7; no. 2; pp. 239 - 248
• Main Authors: Thawer, S G, Horsnell, W GC, Darby, M, Hoving, J C, Dewals, B, Cutler, A J, Lang, D, Brombacher, F
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01-03-2014
• Subjects: 631/250/1619/554/1898; 631/250/2152/1566/1571; 631/250/255/1715; 631/326/417/2546; Allergology; Animals; Antibodies; Biomedical and Life Sciences; Biomedicine; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - metabolism; Cell Movement - genetics; Cell Movement - immunology; Disease Models, Animal; Gastroenterology; Gene Expression; Immunologic Memory; Immunology; Interleukin-4 Receptor alpha Subunit - genetics; Interleukin-4 Receptor alpha Subunit - metabolism; Lung - immunology; Lung - metabolism; Lung - parasitology; Lymph Nodes - immunology; Lymph Nodes - metabolism; Mice; Mice, Knockout; Nippostrongylus - immunology; Strongylida Infections - genetics; Strongylida Infections - immunology; Strongylida Infections - metabolism; Strongylida Infections - parasitology
• ISSN: 1933-0219; 1935-3456
• DOI: 10.1038/mi.2013.40

Immunity to
Nippostrongylus brasiliensis
reinfection requires pulmonary CD4
+
T-cell responses. We examined whether secondary lymphoid recruited or pre-existing lung CD4
+
T-cell populations coordinated this immunity. To do this, we blocked T-cell egress from lymph nodes using Fingolimod (FTY720). This impaired host ability to resolve a primary infection but did not change effectiveness of recall immunity. Associated with this effective recall immunity was the expansion and T helper type 2 polarization of a pre-existing pulmonary CD4
+
T-cell population. LTβR-Ig (lymphotoxin beta-receptor fusion protein)-mediated disruption of stromal cell organization of immune cells did not disrupt this recall immunity, suggesting that protection was mediated by a pulmonary interstitial residing CD4
+
T-cell population. Adoptive transfer of
N. brasiliensis
-experienced pulmonary CD4
+
T cells from FTY720-treated wild-type or T-cell interleukin (IL)-4Rα-deficient mice demonstrated protection to be IL-4Rα dependent. These results show that pre-existing CD4
+
T cells can drive effective recall immunity to
N. brasiliensis
infection independently of T-cell recruitment from secondary lymphoid organs.