Relationships between composite roughness and Streptococcus mutans biofilm depth under shear in vitro

• Published in: Journal of dentistry Vol. 134; p. 104535
• Main Authors: O'Brien, Evan P., Mondal, Karabi, Chen, Chien-Chia, Hanley, Luke, Drummond, James L., Rockne, Karl J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-07-2023
• Subjects: Bacterial Adhesion; Biofilm; Biofilms; Composite Resins - chemistry; Dental composite; Dental Materials - chemistry; Glucose; Materials Testing; Streptococcus mutans; Sucrose; Sucrose - pharmacology; Surface Properties; Surface roughness; Biofilm; Surface roughness; Glucose; Sucrose; Dental composite; Streptococcus mutans
• ISSN: 0300-5712; 1879-176X
• DOI: 10.1016/j.jdent.2023.104535

To investigate the effect of substrate, surface roughness, and hydraulic residence time (HRT) on Streptococcus mutans biofilms growing on dental composites under conditions relevant to the oral cavity.
Dental composites were prepared with varying amounts of polishing and incubated in a CDC bioreactor with an approximate shear of 0.4 Pa. S. mutans biofilms developed in the bioreactors fed sucrose or glucose and at 10-h or 40-h HRT for one week. Biofilms were characterized by confocal laser microscopy (CLM). Composite surface roughness was characterized by optical profilometry, and pre- and post-incubation composite surface fine structure and elemental composition were determined using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS).
Polishing had a significant impact on surface roughness, varying by a factor of 15 between the polished samples and the unpolished control. S. mutans biofilms grew statistically significantly thicker on the unpolished composites. Biofilm thickness was greater at shorter 10-h HRT compared to 40-h HRT. In most cases, biofilm thickness was not statistically significantly greater in sucrose-fed bioreactors than in glucose-fed bioreactors. SEM-EDS analysis did not identify any significant change in elemental composition after aging.
Accurate characterization of oral cavity biofilms must consider shear forces and the use of techniques that minimize alteration of the biofilm structure. Under shear, surface smoothness is the most important factor determining S. mutans biofilm thickness followed by HRT, while sucrose presence did not result in significantly greater biofilm thickness.
The obvious patterns of S. mutans growth along sub-micron scale grooving created by the polishing process suggested that initial biofilm attachment occurred in the shear-protected grooves. These results suggest that fine polishing may help prevent the initial formation of S. mutans biofilms compared to unpolished/coarse polished composites.