Photodynamic therapry with curcumin in the reduction of enterococcus faecalis biofilm in bone cavity: rMicrobiological and spectral fluorescense analysis

• Published in: Photodiagnosis and photodynamic therapy Vol. 33; p. 102084
• Main Authors: Rocha, Marisol Porto, Santos, Mariana Sousa, Rodrigues, Paôlla Layanna Fernandes, Araújo, Thalita Santos Dantas, de Oliveira, Janeide Muritiba, Rosa, Luciano Pereira, Bagnato, Vanderlei Salvador, da Silva, Francine Cristina
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-03-2021
• Subjects: Animals; Antimicrobial; Biofilms; Blue LED; Bovine bone; Cattle; Curcumin - pharmacology; Enterococcus faecalis; Photochemotherapy - methods; Photodynamic therapy; Photosensitizing Agents - pharmacology; Enterococcus faecalis; Antimicrobial; Blue LED; Bovine bone; Photodynamic therapy
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2020.102084

•Bactericide effect of PDT was demonstrated in vitro on E. faecalis biofilm.•A positive correlation between the amount of red pixels in the image obtained by fluorescence spectroscopy and the presence of biofilm was demonstrated.•The findings are of importance to guide future clinical studies in patients with intra-osseous lesions of dental interest and other types of osteomyelitis.
Antimicrobial photodynamic therapy (PDT) has emerged as a therapeutic strategy to conventional procedures using antibiotics.
To evaluate the antimicrobial effectiveness of PDT using blue light emitting diode (LED) associated with curcumin on biofilms of Enterococcus faecalis in bovine bone cavities and also to analyze the presence of these biofilms through spectral fluorescence.
Standardized suspensions of E. faecalis (ATCC 29212) were incubated in artificial bone cavities for 14 days at 36 °C ± 1 °C for biofilm formation. The test specimens were distributed among the four experimental groups (n = 10): L-C- (control), L + C- (LED for 5 min), L-C+ (curcumin for 5 min) and L + C+ (PDT). Aliquots were collected from the bone cavities after treatments and seeded on BHI agar for 24 h at 36 °C ± 1 °C for CFU count. Before and after each treatment the specimens were submitted to spectral fluorescence, whose images were compared in the Image J program. The log10 CFU/mL results were submitted to the Kruskal–Wallis test (5%) and the biofilm fluorescence spectroscopy results were submitted to the Wilcoxon test (5%).
All treatments presented statistical difference when compared to the control, and PDT was responsible for the largest reduction (1.92 log10 CFU/mL). There was a reduction in the fluorescence emitted after the treatments, with greater statistical difference in the PDT group.
PDT was efficient in the reduction of E. faecalis biofilms. In all groups post treatment there was a significant reduction of biofilms in the fluorescence spectroscopy images with greater reduction in the PDT group.