Cytotoxic activity of Serratia marcescens clinical isolates

Twenty Serratia marcescens isolates from clinical specimens were examined for their cytotoxic activity on four cell lines (HEp-2, Vero, CHO, J774). Most of the isolates were found to be cytotoxic to CHO (70%), Vero (75%) and HEp-2 cells (90%). CHO cells were the most sensitive to cell-free supernata...

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Bibliographic Details
Published in:	Polish journal of microbiology Vol. 59; no. 3; pp. 201 - 205
Main Authors:	Krzymińska, Sylwia, Raczkowska, Marta, Kaznowski, Adam
Format:	Journal Article
Language:	English
Published:	Poland De Gruyter Poland 2010
Subjects:	Animals Cercopithecus aethiops CHO Cells Clinical isolates Cricetinae Cricetulus Cytotoxicity Hep G2 Cells Humans Macrophages - microbiology Mice Serratia Infections - microbiology Serratia marcescens - physiology Vero Cells
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Summary:	Twenty Serratia marcescens isolates from clinical specimens were examined for their cytotoxic activity on four cell lines (HEp-2, Vero, CHO, J774). Most of the isolates were found to be cytotoxic to CHO (70%), Vero (75%) and HEp-2 cells (90%). CHO cells were the most sensitive to cell-free supernatants, followed by HEp-2 and Vero cells. Two strains produced cytotonic toxins which caused elongation of CHO cells. Moreover, twelve isolates (60%) revealed cytotoxic potential to macrophage cell line J774. The results indicate that these bacteria may destroy phagocytes and epithelial cells, which may lead to spread within the host.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1733-1331 2544-4646
DOI:	10.33073/pjm-2010-031