Metal ion content of internal organs in the calorically restricted Wistar rat

• Published in: Journal of trace elements in medicine and biology Vol. 78; p. 127182
• Main Authors: Santos-Díaz, Alma I., Solís-López, Jordi, Díaz-Torres, Elizabeth, Guadarrama-Olmos, José C., Osorio, Beatriz, Kroll, Thomas, Webb, Samuel M., Hiriart, Marcia, Jiménez-Estrada, Ismael, Missirlis, Fanis
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-07-2023
• Subjects: Animals; Biometals; Calcium; Calcium aggregates; Copper; Female; Humans; Inflammation; Male; Manganese; Metallome; Muscle, Skeletal; Python code; Rats; Rats, Wistar; Synchrotron; Zinc; Calcium aggregates; Inflammation; Metallome; Python code; Synchrotron; Biometals
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/j.jtemb.2023.127182

Despite the agreed principle that access to food is a human right, undernourishment and metal ion deficiencies are public health problems worldwide, exacerbated in impoverished or war-affected areas. It is known that maternal malnutrition causes growth retardation and affects behavioral and cognitive development of the newborn. Here we ask whether severe caloric restriction leads per se to disrupted metal accumulation in different organs of the Wistar rat.
Inductively coupled plasma optical emission spectroscopy was used to determine the concentration of multiple elements in the small and large intestine, heart, lung, liver, kidney, pancreas, spleen, brain, spinal cord, and three skeletal muscles from control and calorically restricted Wistar rats. The caloric restriction protocol was initiated from the mothers prior to mating and continued throughout gestation, lactation, and post-weaning up to sixty days of age.
Both sexes were analyzed but dimorphism was rare. The pancreas was the most affected organ presenting a higher concentration of all the elements analyzed. Copper concentration decreased in the kidney and increased in the liver. Each skeletal muscle responded to the treatment differentially: Extensor Digitorum Longus accumulated calcium and manganese, gastrocnemius decreased copper and manganese, whereas soleus decreased iron concentrations. Differences were also observed in the concentration of elements between organs independently of treatment: The soleus muscle presents a higher concentration of Zn compared to the other muscles and the rest of the organs. Notably, the spinal cord showed large accumulations of calcium and half the concentration of zinc compared to brain. X-ray fluorescence imaging suggests that the extra calcium is attributable to the presence of ossifications whereas the latter finding is attributable to the low abundance of zinc synapses in the spinal cord.
Severe caloric restriction did not lead to systemic metal deficiencies but caused instead specific metal responses in few organs.
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•The pancreas concentrates metal ions in the calorically restricted rat.•Copper shifts from kidney to liver upon caloric restriction.•Skeletal muscles show differential responses to caloric restriction.•Calcium aggregates and low abundance of zinc synapses in the spinal cord.