Genomic comparison of 60 completely sequenced bacteriophages that infect Erwinia and/or Pantoea bacteria

• Published in: Virology (New York, N.Y.) Vol. 535; pp. 59 - 73
• Main Authors: Thompson, Daniel W., Casjens, Sherwood R., Sharma, Ruchira, Grose, Julianne H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-09-2019
• Subjects: Bacteriophage; Bacteriophages; Bacteriophages - genetics; Bacteriophages - isolation & purification; Cluster; Cluster Analysis; Diversity; Enterobacteriales; Erwinia; Erwinia - virology; Erwiniaceae; Genome, Viral; Genomics; Pantoea; Pantoea - virology; Phage; Phylogeny; Whole Genome Sequencing; Bacteriophages; Erwinia; Diversity; Pantoea; Cluster; Enterobacteriales; Bacteriophage; Erwiniaceae; Phage
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2019.06.005

Erwinia and Pantoea are closely related bacterial plant pathogens in the Gram negative Enterobacteriales order. Sixty tailed bacteriophages capable of infecting these pathogens have been completely sequenced by investigators around the world and are in the current databases, 30 of which were sequenced by our lab. These 60 were compared to 991 other Enterobacteriales bacteriophage genomes and found to be, on average, just over twice the overall average length. These Erwinia and Pantoea phages comprise 20 clusters based on nucleotide and protein sequences. Five clusters contain only phages that infect the Erwinia and Pantoea genera, the other 15 clusters are closely related to bacteriophages that infect other Enterobacteriales; however, within these clusters the Erwinia and Pantoea phages tend to be distinct, suggesting ecological niche may play a diversification role. The failure of many of their encoded proteins to have predicted functions highlights the need for further study of these phages.