Immunohistochemical quantitation for extracellular matrix proteins in rats with glomerulonephritis induced by monoclonal anti-Thy-1.1 antibody

• Published in: Nephron (2015) Vol. 71; no. 1; p. 79
• Main Authors: Manabe, N, Furuya, Y, Nagano, N, Yagi, M, Kuramitsu, K, Miyamoto, H
• Format: Journal Article
• Language: English
• Published: Switzerland 1995
• Subjects: Animals; Disease Models, Animal; Extracellular Matrix Proteins - metabolism; Glomerulonephritis - chemically induced; Glomerulonephritis - metabolism; Glomerulonephritis - pathology; Immunohistochemistry; Isoantibodies; Kidney Cortex - metabolism; Male; Random Allocation; Rats; Rats, Inbred Lew
• DOI: 10.1159/000188678
• ISSN: 1660-8151

Extracellular matrix proteins (type I collagen and fibronectin) in frozen histologic sections of kidney cortex from rats with glomerulonephritis induced by a single intravenous administration of anti-Thy 1.1 antibody were quantified using an immunohistochemical micromethod. Type I collagen and fibronectin contents in renal cortex of rats with experimental glomerulonephritis (4.33 +/- 0.79 and 10.41 +/- 2.01 microgram/mg of total protein, respectively) were 262% and 151%, respectively, higher than in control rats given normal mouse IgG (1.65 +/- 0.16 and 6.88 +/- 0.95 microgram/mg, respectively; p < 0.01 in each case). In the glomerulonephritic rats, the increase in the contents of extracellular matrix proteins, especially type I collagen, correlated with increasing glomeruli with expansion of mesangial areas. The increase in type I collagen content correlated well with increasing urinary protein excretion and blood urea nitrogen and serum total cholesterol levels (r = 0.851, 0.812, and 0.837, respectively; p < 0.05 in each case). The decrease in creatinine clearance correlated with increasing content of type I collagen (r = 0.781; p < 0.05). The immunohistochemical micromethod may make it possible to evaluate the histopathological diagnosis of mesangial proliferative glomerulonephritis quantitatively.