A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples

• Published in: Revista da Sociedade Brasileira de Medicina Tropical Vol. 43; no. 6; pp. 728 - 730
• Main Authors: Koishi, Andrea Cristine, Vituri, Débora Fonseca, Dionízio Filho, Pedro Sebastião Raimundo, Sasaki, Alexandre Augusto, Felipe, Maria Sueli Soares, Venancio, Emerson José
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Medicina Tropical 01-11-2010; Sociedade Brasileira de Medicina Tropical - SBMT
• Subjects: Adult; DNA, Fungal - analysis; Humans; Male; Middle Aged; Mouth Diseases - diagnosis; Mouth Diseases - microbiology; Paracoccidioides - genetics; Paracoccidioides - isolation & purification; Paracoccidioidomycosis - diagnosis; Paracoccidioidomycosis - microbiology; Polymerase Chain Reaction - methods; Sensitivity and Specificity; TROPICAL MEDICINE; Vector-borne diseases; Molecular detection; Semi-nested PCR; Detecção molecular; Paracoccidioides brasiliensis
• ISSN: 0037-8682; 1678-9849; 1678-9849; 0037-8682
• DOI: 10.1590/S0037-86822010000600026

Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis.
In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were used in the second reaction. The semi-nested PCR was used to investigate biopsies of five patients with oral lesions that resembled paracoccidioidomycosis.
The semi-nested PCR was positive for four samples and negative for a sample from a patient later diagnosed with leishmaniasis.
The new semi-nested PCR describe is useful for paracoccidioidomycosis diagnosis.