Integration of photosynthetic biosensor with molecularly imprinted polymer-based solid phase extraction cartridge

• Published in: Analytica chimica acta Vol. 569; no. 1; pp. 50 - 57
• Main Authors: Breton, F., Euzet, P., Piletsky, S.A., Giardi, M.T., Rouillon, R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 31-05-2006; Elsevier
• Subjects: Analytical chemistry; Applied sciences; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Exact sciences and technology; Extraction; Fluorescence; Fundamental and applied biological sciences. Psychology; General, instrumentation; Global environmental pollution; Herbicides; Methods. Procedures. Technologies; Molecularly imprinted polymer; Photosynthetic biosensor; Pollution; Spectrometric and optical methods; Spinacia oleracea; Various methods and equipments; Fluorescence; Herbicides; Extraction; Photosynthetic biosensor; Molecularly imprinted polymer; Water; Solid phase extraction; Molecular structure; Pesticides; Polymer; Selectivity; Chemical sensor; Herbicide; Fluorescence spectrometry; Environmental control; Chemical enrichment; Sensitivity; Sample preparation; Biosensor; Metribuzin; Regulation; Inhibition; Molecular imprinting; Atrazine
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2006.03.086

A simple method for the detection of individual groups of photosynthesis-inhibiting herbicides was proposed as a practical approach for environmental monitoring. The detection method is based on fluorescent measurement of the inhibition of spinach thylakoidal activity by herbicides. The sensor selectivity was improved by combining biosensor with a sample preconcentration based on a SPE cartridge packed with a polymer, imprinted with cyanazine. The combination of biosensor with molecularly imprinted polymer (MIP) allows selective measurement of triazines herbicides (cyanazine, atrazine) and their discrimination from phenylurea (diuron) or triazinone (metribuzin) herbicides. This combination also helps improving the sensitivity of the measurement allowing the detection of photosynthesis-inhibiting herbicides in water at the level required by European Union regulations.