Bacoside A downregulates matrix metalloproteinases 2 and 9 in DEN-induced hepatocellular carcinoma

• Published in: Cell biochemistry and function Vol. 28; no. 2; pp. 164 - 169
• Main Authors: Janani, Panneerselvam, Sivakumari, Kanakarajan, Geetha, Arumugam, Yuvaraj, Sambandam, Parthasarathy, Chandrakesan
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-03-2010
• Subjects: Animals; bacoside A; Diethylnitrosamine - toxicity; Down-Regulation; hepatocelluar carcinoma; Liver Neoplasms, Experimental - chemically induced; Liver Neoplasms, Experimental - enzymology; Male; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; MMP-2; MMP-9; n-nitroso diethylamine; Rats; Rats, Wistar; Saponins - pharmacology; Triterpenes - pharmacology
• ISSN: 0263-6484; 1099-0844
• DOI: 10.1002/cbf.1638

Cancer metastasis is a complex multi‐step process, responsible for a majority of cancer‐related deaths by affecting the critical organs and causing complications in therapies. Hepatocellular carcinoma is a multi‐factorial disease and is the third most common cause of cancer related mortality worldwide. Clinical and experimental studies have shown that MMP‐2 and MMP‐9 are involved in tumor invasion and metastases and their elevated expression has been associated with poor prognosis. Our recent studies showed a strong anti‐oxidant and hepatoprotective effects of bacoside A (BA) against carcinogen. Nevertheless the effect of BA on the activities and expression of MMP‐2 and MMP‐9 during hepatocellular carcinoma is not yet recognized. Therefore, the present study was designed to assess the same. Results of gelatin zymography study showed that BA co‐treatment significantly decreased the activities of MMP‐2 and MMP‐9, which is increased during hepatocellular carcinoma. Further immunoblot analysis showed decreased expression of MMP‐2 and MMP‐9 in rats co‐treated with BA compared to DEN‐induced hepatocellular carcinoma. Our results reveal that BA exerts its anti‐metastatic effect against DEN‐induced hepatocellular carcinoma by inhibiting the activities and expressions of MMP‐2 and MMP‐9. Copyright © 2010 John Wiley & Sons, Ltd.