Adenoviral Gene Delivery from Multilayered Polyelectrolyte Architectures

• Published in: Advanced functional materials Vol. 17; no. 2; pp. 233 - 245
• Main Authors: Dimitrova, M., Arntz, Y., Lavalle, P., Meyer, F., Wolf, M., Schuster, C., Haïkel, Y., Voegel, J.-C., Ogier, J.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 22-01-2007; WILEY‐VCH Verlag
• Subjects: Gene delivery; Layer-by-layer assembly; Polyelectrolytes; Thin films
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.200600155

The alternate layer‐by‐layer (LBL) deposition of polycations and polyanions for the build up of multilayered polyelectrolyte films is an original approach that allows the preparation of tunable, biologically active surfaces. The resulting supramolecular nanoarchitectures can be functionalized with drugs, peptides, and proteins, or DNA molecules that are able to transfect cells in vitro. We monitor, for the first time, the embedding of a bioactive adenoviral (Ad) vector in multilayered polyelectrolyte films. Ad efficiently adsorbs on poly(L‐lysine)–poly(L‐glutamic acid) (PLL–PGA), PLL–HA (HA: hyaluronan), poly(allylamin hydrochloride)–poly(sodium‐4‐styrenesulfonate) (PAH–PSS), and CHI–HA (CHI: chitosan) films; it preserves its transduction capacity (which can reach 95 %) for a large number of cell types, and also allows vector uptake into receptor‐deficient cells, thus abrogating the restricted tropism of Ad.
A bioactive adenoviral vector is embedded into multilayered polyelectrolyte films. Ad efficiently adsorbs on poly(L‐lysine)–poly(L‐glutamic acid), poly(L‐lysine)–hyaluronan, poly(allylamin hydrochloride)–poly(sodium‐4‐styrenesulfonate) (see the AFM image), and chitosan–hyaluronan films, and preserves its transduction capacity for a large number of cell types.