Lab-on-a-Chip Biosensor for Glucose Based on a Packed Immobilized Enzyme Reactor

In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing...

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Published in:	Electroanalysis (New York, N.Y.) Vol. 19; no. 23; pp. 2451 - 2456
Main Authors:	Blanes, Lucas, Mora, Maria F., do Lago, Claudimir L., Ayon, Arturo, García, Carlos D.
Format:	Journal Article
Language:	English
Published:	Weinheim WILEY-VCH Verlag 01-12-2007 WILEY‐VCH Verlag
Subjects:	Capillary electrophoresis Glucose Immobilized enzyme reactor Microchips
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Abstract	In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post‐column IMER that produces H2O2. The H2O2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 μM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip‐CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip‐CE‐IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports.
AbstractList	In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post-column IMER that produces H2O2. The H2O2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 muM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip-CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip-CE-IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports. In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post‐column IMER that produces H 2 O 2 . The H 2 O 2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 μM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip‐CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip‐CE‐IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports. In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post‐column IMER that produces H2O2. The H2O2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 μM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip‐CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip‐CE‐IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports.
Author	Blanes, Lucas García, Carlos D. do Lago, Claudimir L. Ayon, Arturo Mora, Maria F.
Author_xml	– sequence: 1 givenname: Lucas surname: Blanes fullname: Blanes, Lucas organization: Instituto de Química, Universidade de São Paulo. Av. Prof. Lineu Prestes, 748, 05508-900 São Paulo - SP, Brazil – sequence: 2 givenname: Maria F. surname: Mora fullname: Mora, Maria F. organization: Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA – sequence: 3 givenname: Claudimir L. surname: do Lago fullname: do Lago, Claudimir L. organization: Instituto de Química, Universidade de São Paulo. Av. Prof. Lineu Prestes, 748, 05508-900 São Paulo - SP, Brazil – sequence: 4 givenname: Arturo surname: Ayon fullname: Ayon, Arturo organization: Department of Electrical Engineering, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA – sequence: 5 givenname: Carlos D. surname: García fullname: García, Carlos D. email: carlos.garcia@utsa.edu organization: Department of Chemistry, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA
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Snippet	In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The...
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SubjectTerms	Capillary electrophoresis Glucose Immobilized enzyme reactor Microchips
Title	Lab-on-a-Chip Biosensor for Glucose Based on a Packed Immobilized Enzyme Reactor
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