Scanning probe microscopy in microbiology

• Published in: Micron (Oxford, England : 1993) Vol. 26; no. 4; pp. 347 - 362
• Main Authors: Firtel, M., Beveridge, T.J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 1995
• Subjects: Bacteria - ultrastructure; DNA - ultrastructure; Elasticity; Equipment Design; Microbiological Techniques - instrumentation; Microscopy, Atomic Force - instrumentation; Microscopy, Atomic Force - methods; Microscopy, Immunoelectron - methods; Microscopy, Scanning Tunneling - instrumentation; Microscopy, Scanning Tunneling - methods; Viruses - ultrastructure; Viscosity
• ISSN: 0968-4328; 1878-4291
• DOI: 10.1016/0968-4328(95)00012-7

Scanning probe microscopy (SPM) is emerging as an important alternative to electron microscopy as a technique for analyzing submicron details on biological surfaces. Microbiological specimens such as viruses, bacteriophages, and ordered bacterial surface layers and membranes have played an important role in the development of scanning tunnelling microscopy (STM) and atomic force microscopy (AFM) in cellular and molecular biology. Early STM studies involving metal-coated bacteriophage T4 polyheads, Methanospirillum hungatei, and Deinococcus radiodurans HPI layer clearly demonstrated that resolution was comparable to TEM on similarly prepared specimens and only limited by metal graininess. However, except for thin films of layers, novel biological information has been difficult to obtain since imaging native surfaces of such biomaterials as proteins or nucleic acids by STM proved to be unreliable. With the development of atomic force microscopes, which allow imaging of similar native structures, SPM applications have widened to include straightforward surface structure analysis, analysis of surface elastic and inelastic properties, bonding force measurements between molecules, and micro-manipulations of such individual molecules as DNA. AFM images have progressed from relatively crude representations of specimen topography to nanometer scale representations of native hydrated surfaces. It appears from the study of microbiological specimens that direct visualization of dynamic molecular events or processes may soon become a reality.