A rapid miniaturized solid-phase radioimmunoassay technique for secreted immunoglobulins, employing microtiter plates, antibody bound to polyacrylamide beads, and filter strip harvesting

• Published in: Journal of immunological methods Vol. 49; no. 3; p. 261
• Main Authors: Nash, G S, Seiden, M V, Beale, M G, MacDermott, R P
• Format: Journal Article
• Language: English
• Published: Netherlands 26-03-1982
• Subjects: Acrylic Resins - pharmacology; Animals; Binding Sites, Antibody; Cross-Linking Reagents - pharmacology; Electronics; Filtration - instrumentation; Humans; Immunoglobulin A - analysis; Immunoglobulin G - analysis; Immunoglobulin M - analysis; Immunoglobulins - biosynthesis; Miniaturization; Rabbits; Radioimmunoassay - methods; Time Factors
• ISSN: 0022-1759
• DOI: 10.1016/0022-1759(82)90126-0

We have developed a solid-phase radioimmunoassay technique, which allows a large number of samples to be assayed, while minimizing amounts of reagents and technician time. The assay is run in microtiter plates, which results in improved organization, shorter assay set-up time and advantageous miniaturization. On the first day only 3 reagents are required: sample or standard, a 125I-labeled antigen, and an antibody attached to a polyacrylamide bead. After on overnight incubation period, the assay is harvested using a commercial microharvesting apparatus (24 samples/2 min) and placed directly into a gamma counter for counting. In the present study, we have developed the assay to measure secreted IgG, IgM and IgA, and we have compared our results with those of a standard radioimmunoassay technique. This rapid, simple, economical approach should be applicable to the radioimmunoassay of other substances as well.