Augmentation of murine natural killer activity after culture

The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density whe...

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Bibliographic Details
Published in:	Journal of immunological methods Vol. 48; no. 3; p. 359
Main Authors:	Cohen, S A, Salazar, D, Kaminski, D A
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1982
Subjects:	Animals Cells, Cultured Concanavalin A - pharmacology Cytotoxicity Tests, Immunologic - methods Female Lipopolysaccharides - pharmacology Mice Mice, Inbred C3H Spleen - cytology Spleen - immunology
Online Access:	Get more information
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Summary:	The augmentation of murine natural killer (NK) cell activity after culture is described. Increased NK cell activity occurred when spleen cells were cultured for 18 or 42 h at high cell density in macro culture plates at 37 degrees C. Similar results were also achieved using the same cell density when micro culture plates were employed. The simple modifications of culture conditions described in this paper should provide an excellent tool to study murine NK activity after culture. Furthermore, the micro culture system has the added advantage of enabling one to test large numbers of samples.
ISSN:	0022-1759
DOI:	10.1016/0022-1759(82)90336-2