Evidence for γ-actin as a Z disc component in skeletal myofibers
We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged γ-actin in rat flexor digitorum brevis myofibers. We found that the γ-actin fusion proteins accumulated into Z dis...
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| Published in: | Experimental cell research Vol. 315; no. 2; pp. 218 - 225 |
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| Abstract | We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged γ-actin in rat
flexor digitorum brevis myofibers. We found that the γ-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific α-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the γ-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of γ-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells. |
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| AbstractList | We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged γ-actin in rat
flexor digitorum brevis myofibers. We found that the γ-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific α-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the γ-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of γ-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells. We investigated the targeting of the gamma-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein (GFP-) as well as myc-tagged gamma-actin in rat flexor digitorum brevis myofibers. We found that the gamma-actin fusion proteins accumulated into Z discs but not beneath the sarcolemma. Instead, the GFP-tagged skeletal muscle-specific alpha-actin isoform was preferentially incorporated into the pointed ends of thin contractile filaments. The localization pattern of the gamma-actin fusion proteins was completely different from that of the dystrophin glycoprotein complex on the sarcolemma. The results emphasize the role of gamma-actin as a Z disc component but fail to reveal an actin-based sub-sarcolemmal cytoskeleton in skeletal muscle cells. |
| Author | Metsikkö, Kalervo Kaakinen, Mika Kaisto, Tuula Leinonen, Sanna Papponen, Hinni |
| Author_xml | – sequence: 1 givenname: Hinni surname: Papponen fullname: Papponen, Hinni – sequence: 2 givenname: Tuula surname: Kaisto fullname: Kaisto, Tuula – sequence: 3 givenname: Sanna surname: Leinonen fullname: Leinonen, Sanna – sequence: 4 givenname: Mika surname: Kaakinen fullname: Kaakinen, Mika – sequence: 5 givenname: Kalervo surname: Metsikkö fullname: Metsikkö, Kalervo email: kalervo.metsikko@oulu.fi |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19013151$$D View this record in MEDLINE/PubMed |
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| Keywords | Sarcolemma γ-actin Muscle GFP-actin Dystrophin Z disc |
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| Snippet | We investigated the targeting of the γ-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent protein... We investigated the targeting of the gamma-actin isoform in skeletal myofibers. For this purpose we used expression vectors to produce green fluorescent... |
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| SubjectTerms | Actin Cytoskeleton - metabolism Actins - genetics Actins - metabolism Animals Cell Line Dystroglycans - metabolism Dystrophin Female Fluorescence Recovery After Photobleaching GFP-actin Muscle Muscle Fibers, Skeletal - cytology Muscle Fibers, Skeletal - metabolism Myoblasts, Skeletal - cytology Myoblasts, Skeletal - metabolism Myofibrils - metabolism Phalloidine - metabolism Protein Isoforms - metabolism Rats Rats, Sprague-Dawley Recombinant Fusion Proteins - metabolism Sarcolemma Sarcomeres - metabolism Transduction, Genetic Z disc γ-actin |
| Title | Evidence for γ-actin as a Z disc component in skeletal myofibers |
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