Selection of rhizobacteria able to produce metabolites active against Meloidogyne exigua

• Published in: European journal of plant pathology Vol. 119; no. 4; pp. 477 - 479
• Main Authors: Oliveira, Denilson F, Campos, Vicente P, Amaral, Daniel R, Nunes, Alexandro S, Pantaleão, José A, Costa, Deivid A
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-12-2007; Springer; Springer Nature B.V
• Subjects: Bacillus (bacteria); Bacillus cereus; Bacillus megaterium; Bacteria; Bacterial plant pathogens; Biological and medical sciences; Coffea arabica; Coffee; Enterobacter; Fundamental and applied biological sciences. Psychology; Meloidogyne exigua; Metabolites; Microbiology; Nematoda; Paenibacillus macerans; Phytopathology. Animal pests. Plant and forest protection; Pumilus; root-knot nematodes; South America; Brazil; America; Plant pathology; Microbiology; Plant pathogen; Metabolite; Paenibacillus macerans; Bacillaceae; Root-knot nematode; Plant; Bacillus cereus; Enterobacter; Bacillales; Bacillus; Bacteria; Disseminated; Nematoda; Meloidogyne exigua; Enterobacteriaceae; Root; In vitro; Strain; Treatment; Coflea arabica; Helmintha; Nemathelminthia; Invertebrata
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-007-9176-y

This work aimed to select potentially useful rhizobacteria for the control of Meloidogyne exigua, a widely disseminated root-knot nematode found in Brazilian coffee fields. Initially, crude metabolites produced in liquid medium by 69 isolated rhizobacteria strains (previously obtained from several plants collected in the south of Minas Gerais State, Brazil) were tested in vitro for lethality towards M. exigua second stage juveniles (J₂). Substances produced by strains of Bacillus cereus, B. megaterium, B. pumilus, B. thuringiensis, Enterobacter asburiae, E. cloacae and Paenibacillus macerans caused high mortality in J₂. Subsequently, the ten most active crude metabolites were selected and assessed for nematicidal activity in a biotest with M. exigua-inoculated coffee plants. Although strains of B. cereus, B. pumilus and P. macerans caused a significant reduction in the number of root galls and/or nematode eggs per plant 90 days after treatment, the best results were obtained with the application of B. megaterium strain 54-06.