Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1,758)

The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen–thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane...

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Published in:	Microscopy research and technique Vol. 81; no. 11; pp. 1301 - 1309
Main Authors:	Bezerra, Luana Grasiele Pereira, Souza, Ana Liza Paz, Silva, Herlon Victor Rodrigues, Vasconcelos, Fábio Roger, Moura, Arlindo de Alencar Araripe, Pereira, Alexsandra Fernandes, Oliveira, Moacir Franco de, Silva, Alexandre Rodrigues
Format:	Journal Article
Language:	English
Published:	Hoboken, USA John Wiley & Sons, Inc 01-11-2018 Wiley Subscription Services, Inc
Subjects:	Chromatin Damage detection Fibers Freezing freezing–thawing Glycerol Integrity Light microscopy Males Melting Mitochondria Morphology Motility Scanning electron microscopy Semen Sperm Tayassu Tayassu tajacu Thawing Transmission electron microscopy Ultrastructure Yolk scanning electron microscopy semen transmission electron microscopy morphology freezing-thawing Tayassu tajacu
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Abstract	The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen–thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen–thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing–thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries’ sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen–thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing–thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation. Transmission electron microscopy of frozen–thawed sperm from collared peccaries (Pecari tajacu), presence of vesicles (V) around sperm heads demonstrating acrosomal reaction and electron lucent nuclear dot (arrows). Peccaries’ sperm presents a large acrosome occupying about ¾ of the cell's head; Cryopreservation provokes nanometric damage on peccaries’ sperm; Scanning and transmission electron microscopy are useful tool for evaluation of fresh and frozen–thawed sperm.
AbstractList	The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen-thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen-thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing-thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries' sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen-thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing-thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation. The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen–thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen–thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired ( p < .05) by freezing–thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries’ sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen–thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing–thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation. The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen–thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen–thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing–thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries’ sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen–thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing–thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation. Transmission electron microscopy of frozen–thawed sperm from collared peccaries (Pecari tajacu), presence of vesicles (V) around sperm heads demonstrating acrosomal reaction and electron lucent nuclear dot (arrows). Peccaries’ sperm presents a large acrosome occupying about ¾ of the cell's head; Cryopreservation provokes nanometric damage on peccaries’ sperm; Scanning and transmission electron microscopy are useful tool for evaluation of fresh and frozen–thawed sperm. The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen-thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen-thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM. Sperm motility, membrane integrity, and functionality were impaired (p < .05) by freezing-thawing procedures, but sperm morphology, and chromatin integrity evaluated by light microscopy were not significantly affected. The SEM revealed that peccaries' sperm presents a flattened head in a paddle format, measuring 6.07 μm in length and 3.84 μm in width, with a vastus acrosome (4.46 μm). Normal tails measure 38.11 μm, being formed by an extensive midpiece with 15.52 μm in length. In frozen-thawed samples, both SEM and TEM provide us information about damage undetected through light microscopy as the presence of vesicles in the acrosome, loose plasma membrane, vacuolized mitochondria, dense fibers disorganized, and decondensed chromatin. In conclusion, we provide the first description of the sperm ultrasctruture in collared peccaries. Moreover, SEM and TEM help us to identify some nanometric damage provoked by freezing-thawing procedures, thus providing valuable information for the improvement of such important protocols used for biobanking formation.
Author	Silva, Herlon Victor Rodrigues Vasconcelos, Fábio Roger Pereira, Alexsandra Fernandes Oliveira, Moacir Franco de Souza, Ana Liza Paz Moura, Arlindo de Alencar Araripe Bezerra, Luana Grasiele Pereira Silva, Alexandre Rodrigues
Author_xml	– sequence: 1 givenname: Luana Grasiele Pereira surname: Bezerra fullname: Bezerra, Luana Grasiele Pereira organization: Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semiárido – UFERSA – sequence: 2 givenname: Ana Liza Paz surname: Souza fullname: Souza, Ana Liza Paz organization: Post‐Graduation Program in Biotechnology, Universidade Federal do Ceará – UFC – sequence: 3 givenname: Herlon Victor Rodrigues surname: Silva fullname: Silva, Herlon Victor Rodrigues organization: Laboratory of Carnivore Reproduction, Universidade Estadual do Ceará – UECE – sequence: 4 givenname: Fábio Roger surname: Vasconcelos fullname: Vasconcelos, Fábio Roger organization: Laboratory of Animal Physiology, Universidade Federal do Ceará – UFC – sequence: 5 givenname: Arlindo de Alencar Araripe surname: Moura fullname: Moura, Arlindo de Alencar Araripe organization: Laboratory of Animal Physiology, Universidade Federal do Ceará – UFC – sequence: 6 givenname: Alexsandra Fernandes surname: Pereira fullname: Pereira, Alexsandra Fernandes organization: Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semiárido – UFERSA – sequence: 7 givenname: Moacir Franco de surname: Oliveira fullname: Oliveira, Moacir Franco de organization: Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semiárido – UFERSA – sequence: 8 givenname: Alexandre Rodrigues orcidid: 0000-0001-6027-1062 surname: Silva fullname: Silva, Alexandre Rodrigues email: legio2000@yahoo.com organization: Laboratory of Animal Germplasm Conservation, Universidade Federal Rural do Semiárido – UFERSA
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CitedBy_id	crossref_primary_10_1016_j_ijbiomac_2022_07_106 crossref_primary_10_3390_ani13091483 crossref_primary_10_1016_j_cryobiol_2019_10_195 crossref_primary_10_1017_S0967199419000868 crossref_primary_10_1016_j_anireprosci_2022_107029 crossref_primary_10_3390_ani13121955
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Keywords	scanning electron microscopy semen transmission electron microscopy morphology freezing-thawing Tayassu tajacu
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Snippet	The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and...
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SubjectTerms	Chromatin Damage detection Fibers Freezing freezing–thawing Glycerol Integrity Light microscopy Males Melting Mitochondria Morphology Motility Scanning electron microscopy Semen Sperm Tayassu Tayassu tajacu Thawing Transmission electron microscopy Ultrastructure Yolk
Title	Ultrastructural description of fresh and frozen/thawed sperm derived from collared peccaries (Pecari tajacu Linnaeus, 1,758)
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