Gold Nanoparticle-Functionalized Diatom Biosilica as Label-Free Biosensor for Biomolecule Detection

Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionaliz...

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Published in:	Frontiers in bioengineering and biotechnology Vol. 10; p. 894636
Main Authors:	Chen, Tongtong, Wu, Feifei, Li, Yang, Rozan, Hussein E., Chen, Xiguang, Feng, Chao
Format:	Journal Article
Language:	English
Published:	Frontiers Media S.A 27-05-2022
Subjects:	antibody Bioengineering and Biotechnology biosensor biosilica diatom gold nanoparticles photoluminescence
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Abstract	Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionalized diatom frustules was developed. Gold nanoparticles (AuNPs) deposited on poly-dopamine-coated diatom frustules via in situ deposition which considerably decreased the intrinsic blue PL intensity of diatom biosilica. Then, goat anti-rabbit immunoglobulin G (IgG) was added to functionalize diatom biosilica-poly-dopamine-AuNPs (DBs-PDA-AuNPs). PL studies revealed that the specific binding with antigen rabbit IgG increased the peak intensity of PL in comparison with the non-complimentary antigen (human IgG). The enhancement in PL intensity of DBs-PDA had a linear correlation with antigen (rabbit IgG) concentration, whose limit of detection (LOD) reached 8 × 10 -6 mg/ml. Furthermore, PL detection based on DBs-PDA-AuNPs showed a high detection sensitivity with the LOD as low as 8 × 10 -9 mg/ml and spread over almost eight orders of magnitude, making it suitable for the sensitive quantitative analysis of immune complex compared with traditional fluorescence immunoassay. Hence, the study proves that the AuNP-functionalized diatom frustules can serve as an effective biosensor platform for label-free PL-based immunoassay.
AbstractList	Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionalized diatom frustules was developed. Gold nanoparticles (AuNPs) deposited on poly-dopamine-coated diatom frustules via in situ deposition which considerably decreased the intrinsic blue PL intensity of diatom biosilica. Then, goat anti-rabbit immunoglobulin G (IgG) was added to functionalize diatom biosilica-poly-dopamine-AuNPs (DBs-PDA-AuNPs). PL studies revealed that the specific binding with antigen rabbit IgG increased the peak intensity of PL in comparison with the non-complimentary antigen (human IgG). The enhancement in PL intensity of DBs-PDA had a linear correlation with antigen (rabbit IgG) concentration, whose limit of detection (LOD) reached 8 × 10 -6 mg/ml. Furthermore, PL detection based on DBs-PDA-AuNPs showed a high detection sensitivity with the LOD as low as 8 × 10 -9 mg/ml and spread over almost eight orders of magnitude, making it suitable for the sensitive quantitative analysis of immune complex compared with traditional fluorescence immunoassay. Hence, the study proves that the AuNP-functionalized diatom frustules can serve as an effective biosensor platform for label-free PL-based immunoassay. Diatom biosilica (DBs) is the cell wall of natural diatom called frustule, which is made of porous hydrogenated amorphous silica possessing periodic micro- to nanoscale features. In this study, a simple, sensitive, and label-free photoluminescence (PL) immune-detection platform based on functionalized diatom frustules was developed. Gold nanoparticles (AuNPs) deposited on poly-dopamine-coated diatom frustules via in situ deposition which considerably decreased the intrinsic blue PL intensity of diatom biosilica. Then, goat anti-rabbit immunoglobulin G (IgG) was added to functionalize diatom biosilica-poly-dopamine-AuNPs (DBs-PDA-AuNPs). PL studies revealed that the specific binding with antigen rabbit IgG increased the peak intensity of PL in comparison with the non-complimentary antigen (human IgG). The enhancement in PL intensity of DBs-PDA had a linear correlation with antigen (rabbit IgG) concentration, whose limit of detection (LOD) reached 8 × 10-6 mg/ml. Furthermore, PL detection based on DBs-PDA-AuNPs showed a high detection sensitivity with the LOD as low as 8 × 10-9 mg/ml and spread over almost eight orders of magnitude, making it suitable for the sensitive quantitative analysis of immune complex compared with traditional fluorescence immunoassay. Hence, the study proves that the AuNP-functionalized diatom frustules can serve as an effective biosensor platform for label-free PL-based immunoassay.
Author	Wu, Feifei Rozan, Hussein E. Chen, Xiguang Li, Yang Chen, Tongtong Feng, Chao
AuthorAffiliation	2 College of Life Sciences , Qingdao University , Qingdao , China 3 Department of Biochemistry , Faculty of Agriculture , Al-Azhar University , Cairo , Egypt 1 College of Marine Life Science , Ocean University of China , Qingdao , China 4 Qingdao National Laboratory for Marine Science and Technology , Qingdao , China
AuthorAffiliation_xml	– name: 1 College of Marine Life Science , Ocean University of China , Qingdao , China – name: 3 Department of Biochemistry , Faculty of Agriculture , Al-Azhar University , Cairo , Egypt – name: 4 Qingdao National Laboratory for Marine Science and Technology , Qingdao , China – name: 2 College of Life Sciences , Qingdao University , Qingdao , China
Author_xml	– sequence: 1 givenname: Tongtong surname: Chen fullname: Chen, Tongtong – sequence: 2 givenname: Feifei surname: Wu fullname: Wu, Feifei – sequence: 3 givenname: Yang surname: Li fullname: Li, Yang – sequence: 4 givenname: Hussein E. surname: Rozan fullname: Rozan, Hussein E. – sequence: 5 givenname: Xiguang surname: Chen fullname: Chen, Xiguang – sequence: 6 givenname: Chao surname: Feng fullname: Feng, Chao
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Title	Gold Nanoparticle-Functionalized Diatom Biosilica as Label-Free Biosensor for Biomolecule Detection
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