Polyphenol analysis using high‐resolution mass spectrometry allows differentiation of drought tolerant peanut genotypes

• Published in: Journal of the science of food and agriculture Vol. 100; no. 2; pp. 721 - 731
• Main Authors: Juliano, Fernanda F, Alvarenga, José Fernando R, Lamuela‐Raventos, Rosa Maria, Massarioli, Adna P, Lima, Liziane M, Santos, Roseane C, Alencar, Severino M
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 30-01-2020; John Wiley and Sons, Limited
• Subjects: Acids; Arachis - chemistry; Arachis - genetics; Arachis - metabolism; Arachis hypogaea; Arachis hypogaea L; Brazil; Chromatography, High Pressure Liquid - methods; Coumaric Acids; Cultivation; Drought resistance; Droughts; Flavanols; Flavonoids; Flavonoids - chemistry; Flavonoids - metabolism; Flavonols; Genotype; Genotypes; Hydroxycinnamic acid; Ionization; Ions; LC‐MS/MS; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Multivariate analysis; Nuts; Organic chemistry; Peanuts; Phenolic compounds; Phenols; Polyphenols; Polyphenols - chemistry; Polyphenols - metabolism; Scientific imaging; Spectrometry, Mass, Electrospray Ionization - methods; Spectroscopy; Water - analysis; Water - metabolism; Water stress; Brazil; phenolic compounds; LC-MS/MS; Arachis hypogaea L; multivariate analysis
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.10075

BACKGROUND
Peanut (Arachis hypogaea L.) is an important economic food crop highly appreciated worldwide. Although peanut cultivation has been expanding to dry regions, with water stress during growth stages, current genotypes are not adapted to drought. This study aimed to identify and quantify the full range of polyphenols in five peanut genotypes with different degrees of drought tolerance developed by Embrapa (Brazil) using ultra‐high‐definition accurate‐mass liquid chromatography–electrospray ionization–LTQ‐Orbitrap–mass spectrometry.
RESULTS
Fifty‐eight polyphenols of the classes hydroxybenzoic acids, hydroxycinnamic acids, flavanols, flavonols and flavanones were identified and quantified using high mass accuracy data and confirmed by tandem mass spectrometric experiments. High‐definition mass spectrometric analyses revealed nine phenolic compounds that have never been reported in peanuts before. Polyphenol assessment using multivariate analysis allowed identification of the level of similarities among the five peanut genotypes studied.
CONCLUSION
Higher drought‐tolerant genotypes exhibited higher content of flavonoids, which suggests a relationship between these compounds and drought tolerance traits. © 2019 Society of Chemical Industry