Electrophoretic characterization of pyrimidine 5'-nucleotidase of human erythrocytes and its distinction from acid phosphatase

Electrophoretic characterization of pyrimidine 5'-nucleotidase of human erythrocytes and its distinction from acid phosphatase

Electrophoresis of red cell pyrimidine 5'-nucleotidase was carried out on cellulose acetate strips. One major band of activity was found in preparations from human erythrocytes. This enzyme showed a specificity for the pyrimidine nucleotides UMP and CMP. No activity was detected with AMP. Pyrim...

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Bibliographic Details
Published in:Clinica chimica acta Vol. 79; no. 1; p. 115
Main Authors: Rosa, R, Valentin, C, Rosa, J
Format: Journal Article
Language:English
Published: Netherlands 15-08-1977
Subjects:
Acid Phosphatase - blood
Adult
Electrophoresis, Cellulose Acetate
Erythrocytes - enzymology
Fetal Blood - enzymology
Humans
Infant, Newborn
Nucleotidases - blood
Pyrimidine Nucleotides
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Summary:Electrophoresis of red cell pyrimidine 5'-nucleotidase was carried out on cellulose acetate strips. One major band of activity was found in preparations from human erythrocytes. This enzyme showed a specificity for the pyrimidine nucleotides UMP and CMP. No activity was detected with AMP. Pyrimidine 5'-nucleotidase activity could be separated from that of acid phosphatase with the use of alpha-glycerophosphate. This method may be useful in the study of pyrimidine 5'-nucleotidase deficiency in red cells.
ISSN:0009-8981
DOI:10.1016/0009-8981(77)90468-5