Multiple biomarkers response of Astyanax lacustris (Teleostei: Characidae) exposed to manganese and temperature increase

• Published in: Environmental toxicology and pharmacology Vol. 100; p. 104124
• Main Authors: Gnocchi, Karla Giavarini, Boldrini-França, Johara, Passos, Larissa Souza, Gomes, Aline Silva, Coppo, Gabriel Carvalho, Pereira, Tatiana Miura, Chippari-Gomes, Adriana Regina
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-2023
• Subjects: Bioaccumulation; Fish; Gene expression; Genotoxicity; Metal; Fish; Metal; Gene expression; Bioaccumulation; Genotoxicity
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2023.104124

The present study aimed to evaluate the toxicity of Mn (6.65 mg/L) at different exposure times (96 h, 7, 14, and 21 days) and evaluate its possible toxic effects on the fish Astyanax lacustris through multi-biomarkers and the maximum critical temperature (CT Max). The results show an increase in the Mn accumulation (liver and gills) with increasing exposure time. The glutathione S-transferase (GST) activity showed differences in the group exposed to Mn for 96 h compared to the group exposed for 21 days. The acetylcholinesterase (AChE) activity increased in the fish exposed for 7 days compared to the control group. On the other hand, no genotoxic changes were observed. The CT Max showed that the loss of equilibrium of 50% of the fish occurs at a temperature of 39ºC, with and without the Mn presence. Furthermore, the catalase gene expression (oxidative stress) did not show alterations.
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•Evaluation of Mn toxicity associated with temperature increase in Astyanax lacustris.•Bioaccumulation of Mn in the gills and liver of fish at different exposure times.•Acetylcholinesterase activity increased in fish exposed for 7 days to Mn.•No genotoxic changes in erythrocytes were observed in fish exposed to Mn.•Mn and temperature increase did not cause alteration in catalase gene expression.