Genetic polymorphisms rs1800871 and rs1800872 of IL-10 gene are associated with dengue infection, especially with serotype 1 and DwoWS in Mexican population

• Published in: Cytokine (Philadelphia, Pa.) Vol. 166; p. 156194
• Main Authors: Eloisa Monroy-Muñoz, Irma, Esteban Muñoz-Medina, José, Manuel Fragoso, José, Esperanza Santacruz-Tinoco, Clara, Sevilla-Montoya, Rosalba, Hidalgo-Bravo, Alberto, Montes-Herrera, Daniel, Bravo-Flores, Eyerahi, Angeles-Martínez, Javier
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-06-2023
• Subjects: Dengue; Dengue - genetics; Genetic Predisposition to Disease; Humans; IL-10; Inflammation; Interleukin-10 - genetics; Polymorphism, Single Nucleotide - genetics; Polymorphisms; Serogroup; SD; DHF; OR; SNP; Dengue; DENV; LD; Inflammation; Polymorphisms; IL-10; DwoWS; DwWS
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2023.156194

•Two IL-10 polymorphisms were analyzed in 365 individuals with Dengue infection and 364 controls.•Independent association of the T and A alleles of the polymorphic sites rs1800871 and rs1800872, with dengue infection.•Independent association of the T and A alleles of the polymorphic sites with serotype 1, and in participants with DwoWS.•Individuals with DwWS presented high levels of IL-10 compared with DwoWS individuals.•Correlation between IL-10 and INF-γ levels with the host genetic component in patients with DwoWS.
Dengue infection is generated by a complex interaction between DENV (Dengue Virus) and the host's immune response. Interleukin-10 is an immunoregulatory cytokine during DENV infection. The objective of this study was to investigate whether genetic variants in IL-10 could be useful as a predictive and susceptibility marker in the prognosis of DENV infection, particularly with serotype 1, and in participants with dengue without warning signs.
A study of cases (n = 365) and controls (n = 364) was carried out. Genotyping was performed by real-time PCR using TaqMan probes. Sample size power was calculated using Quanto software
This is the first report showing the independent association of the T allele of rs1800871 (P = 0.023) and the A allele of rs1800872 (P = 0.010) with the risk of dengue infection. Statistical analysis established the genotypic association of IL-10 SNPs with DENV infection under different inheritance models. Our results also showed the association of the CC, TC, and CA haplotypes (P = 0.0064, P = 0.0032, and P = 0.0010 respectively) with infection. Furthermore, both polymorphic sites were associated with the risk of DwoWS and serotype 1 (Den-1) under different inheritance models. Finally, under the dominant model, we identified a positive correlation between IL-10 levels vs. IFN-γ and IL-8.
Our results show the first independent association of the T and A alleles of the polymorphic sites rs1800871 and rs1800872, with dengue infection, particularly with Den-1, and in participants with DwoWs.