Concentrations of antibodies against β-amyloid 40/42 monomer and oligomers in Chinese intravenous immunoglobulins

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 138; pp. 277 - 282
• Main Authors: Ye, Shengliang, Zeng, Renyong, Jiang, Peng, Hou, Mingxia, Liu, Fengjuan, Wang, Zongkui, Du, Xi, Yuan, Jing, Chen, Yunhua, Cao, Haijun, Ma, Li, Li, Changqing
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 10-05-2017
• Subjects: Alzheimer Disease - immunology; Alzheimer's disease; amyloid; Amyloid beta-Peptides - immunology; Antibodies - immunology; Antibody; beta; China; Enzyme-Linked Immunosorbent Assay - methods; Immunoglobulins, Intravenous - immunology; Intravenous immunoglobulin; Monomer; Oligomers; Peptide Fragments - immunology; Oligomers; amyloid; Antibody; Intravenous immunoglobulin; Monomer; Alzheimer's disease; beta
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2017.02.024

•Anti-Aβ antibody levels in Chinese IVIgs were markedly higher than in Octagam®.•Chinese IVIgs have different antibody concentrations to different Aβ conformations.•The effects of Chinese IVIg products in AD patients should be investigated.
Intravenous immunoglobulin (IVIg) preparations are being investigated as a potential agent for treatment or prevention of Alzheimer’s disease (AD). Antibodies towards soluble β-amyloid (Aβ) contained in IVIg were considered to be the major component contributing to the beneficial effect of the preparations in pilot studies. This study compared the antibody concentrations against Aβ in Octagam® IVIg (Octapharma) and 9 IVIg preparations from different Chinese manufacturers by ELISA, using Aβ40 monomer, Aβ40 soluble oligomers, Aβ42 monomer and Aβ42 soluble oligomers as the antigens. The results showed that each preparation contained different antibody levels against the four Aβ forms. The median values of the four antibody concentrations in Chinese IVIg preparations were 16.53, 8.47, 24.36 and 33.25μg/mL, which were remarkably higher than that in Octagam® IVIg (1.66, 2.07, 4.61 and 4.64μg/mL). Moreover, the anti-Aβ42 oligomer antibody levels in almost all IVIg preparations were higher than the anti-Aβ42 monomer antibody, and the concentrations of anti-Aβ42 antibodies in most of the IVIg preparations were significantly higher than that of anti-Aβ40 antibodies. These findings will contribute to an increased understanding of the uniqueness of Chinese IVIg preparations, and could provide support for a trial of a Chinese IVIg product in AD patients.