Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma
BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs. OBJECTIVEThe purpose of this study was to evaluate the effects of LMWH-P M/N...
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| Published in: | Annals of plastic surgery Vol. 78; no. 5; pp. 562 - 568 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
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01-05-2017
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| Abstract | BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.
OBJECTIVEThe purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).
METHODSA total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.
RESULTSTreatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.
CONCLUSIONSThe prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site. |
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| AbstractList | BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.
OBJECTIVEThe purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).
METHODSA total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.
RESULTSTreatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.
CONCLUSIONSThe prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site. Activated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs. The purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG). A total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days. Treatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site. The prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site. BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.OBJECTIVEThe purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).METHODSA total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.RESULTSTreatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.CONCLUSIONSThe prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site. |
| Author | Takikawa, Makoto Hattori, Hidemi Takabayashi, Yuki Nakamura, Shingo Kiyosawa, Tomoharu Kuwabara, Masahiro Ishihara, Masayuki |
| AuthorAffiliation | From the Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa; †Division of Biomedical Engineering, Research Institute, and ‡Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan |
| AuthorAffiliation_xml | – name: From the Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa; †Division of Biomedical Engineering, Research Institute, and ‡Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan |
| Author_xml | – sequence: 1 givenname: Yuki surname: Takabayashi fullname: Takabayashi, Yuki organization: From the Department of Plastic and Reconstructive Surgery, National Defense Medical College, Tokorozawa; †Division of Biomedical Engineering, Research Institute, and ‡Department of Medical Engineering, National Defense Medical College, Tokorozawa, Saitama, Japan – sequence: 2 givenname: Masayuki surname: Ishihara fullname: Ishihara, Masayuki – sequence: 3 givenname: Masahiro surname: Kuwabara fullname: Kuwabara, Masahiro – sequence: 4 givenname: Makoto surname: Takikawa fullname: Takikawa, Makoto – sequence: 5 givenname: Shingo surname: Nakamura fullname: Nakamura, Shingo – sequence: 6 givenname: Hidemi surname: Hattori fullname: Hattori, Hidemi – sequence: 7 givenname: Tomoharu surname: Kiyosawa fullname: Kiyosawa, Tomoharu |
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| CitedBy_id | crossref_primary_10_1093_jrr_rrx044 crossref_primary_10_1055_s_0041_1734573 crossref_primary_10_1007_s00266_018_1087_0 crossref_primary_10_1016_j_jconrel_2021_12_036 crossref_primary_10_1111_jdv_15391 crossref_primary_10_1097_SCS_0000000000010039 crossref_primary_10_3390_polym14050932 |
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| References | Miller (R22-19-20210203) 2014; 10 Eppley (R10-19-20210203) 2004; 114 Craven (R4-19-20210203) 1999; 40 Seyrek (R19-19-20210203) 2010; 158 Gandhi (R23-19-20210203) 2008; 72 Rice (R3-19-20210203) 1991; 4 Nakamura (R27-19-20210203) 2010; 92 Chen (R13-19-20210203) 2010; 31 Takabayashi (R16-19-20210203) 2015; 193 Kishimoto (R18-19-20210203) 2013; 9 Levine (R2-19-20210203) 1979; 19 Mori (R14-19-20210203) 2010; 5 Danielsen (R25-19-20210203) 2008; 122 Pan (R26-19-20210203) 1997; 80 Fujita (R9-19-20210203) 2013; 101 Ishihara (R20-19-20210203) 2015; 16 Maeda (R5-19-20210203) 1999; 104 Nakamura (R17-19-20210203) 2009; 91A Lynch (R12-19-20210203) 1989; 84 Capla (R6-19-20210203) 2006; 117 Takikawa (R15-19-20210203) 2011; 170 Kishimoto (R28-19-20210203) 2009; 33 Eppley (R7-19-20210203) 2006; 118 Weibrich (R11-19-20210203) 2002; 30 Takikawa (R8-19-20210203) 2011; 97B Nakamura (R24-19-20210203) 2012; 15 Bitsch (R1-19-20210203) 2005; 39 |
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| Snippet | BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs)... Activated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly... |
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| SubjectTerms | Animals Graft Survival - drug effects Heparin, Low-Molecular-Weight - pharmacology Laser-Doppler Flowmetry Male Nanoparticles Platelet-Rich Plasma Protamines - pharmacology Rats Rats, Inbred F344 Skin Transplantation - methods Wound Healing - drug effects |
| Title | Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma |
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