Improved Survival of Full-Thickness Skin Graft With Low-Molecular Weight Heparin-Protamine Micro/Nanoparticles Including Platelet-Rich Plasma

• Published in: Annals of plastic surgery Vol. 78; no. 5; pp. 562 - 568
• Main Authors: Takabayashi, Yuki, Ishihara, Masayuki, Kuwabara, Masahiro, Takikawa, Makoto, Nakamura, Shingo, Hattori, Hidemi, Kiyosawa, Tomoharu
• Format: Journal Article
• Language: English
• Published: United States Copyright Wolters Kluwer Health, Inc. All rights reserved 01-05-2017
• Subjects: Animals; Graft Survival - drug effects; Heparin, Low-Molecular-Weight - pharmacology; Laser-Doppler Flowmetry; Male; Nanoparticles; Platelet-Rich Plasma; Protamines - pharmacology; Rats; Rats, Inbred F344; Skin Transplantation - methods; Wound Healing - drug effects
• ISSN: 0148-7043; 1536-3708
• DOI: 10.1097/SAP.0000000000001051

BACKGROUNDActivated platelet-rich plasma secrets many growth factors (GFs), and low-molecular weight heparin-protamine micro/nanoparticles (LMWH-P M/NPs) significantly interact with, enhance, and stabilize the secreted GFs.
OBJECTIVEThe purpose of this study was to evaluate the effects of LMWH-P M/NPs and GFs (from platelet-rich plasma) on full-thickness skin graft (FTSG).
METHODSA total of 96 inbred male rats were anesthetized and 4-cm full-thickness skin wound were created on dorsal skin of rats. LMWH-P M/NPs and GFs, LMWH-P M/NPs, GFs and saline (control) were then injected evenly into cutaneous muscles at the wound. The next day, the rats underwent FTSG. On the indicated days after FTSG, blood flow of FTSG site (wound bed and FTSG) was examined by 2-dimensional laser Doppler blood flowmeter. On 10 days, pictures of FTSG site were taken and FTSG survival rate was evaluated. Histologic analyses of skin samples were performed on 4, 7, and 10 days.
RESULTSTreatment of full-thickness skin wound with LMWH-P M/NPs and GFs effectively promoted survival rate of FTSG and blood flow of FTSG site compared with those treated with GFs, LMWH-P M/NPs, and control. LMWH-P M/NPs and GFs also promoted new vessel formation at FTSG site.
CONCLUSIONSThe prior injection of LMWH-P M/NPs and GFs into wound bed increases FTSG survival rate, and promotes blood flow and angiogenesis at FTSG site.