Quantitative determination of tolazoline in serum and urine

A high-performance liquid chromatographic procedure is described for the analysis of tolazoline in serum and urine. This assay procedure is suitable for the analysis of micro-samples (50 or 100 microliters serum and 100 microliters urine). Samples are extracted in a single step and injected into a r...

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Bibliographic Details
Published in:	Journal of chromatography Vol. 338; no. 1; p. 123
Main Authors:	Cwik, M J, Chiu, G P, Fischer, J H, Chow-Tung, E, Currie, B L
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1985
Subjects:	Chromatography, High Pressure Liquid Dialysis Humans Hydrolysis Kinetics Tolazoline - analysis Tolazoline - blood Tolazoline - urine
Online Access:	Get more information
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Description
Summary:	A high-performance liquid chromatographic procedure is described for the analysis of tolazoline in serum and urine. This assay procedure is suitable for the analysis of micro-samples (50 or 100 microliters serum and 100 microliters urine). Samples are extracted in a single step and injected into a reversed-phase high-performance liquid chromatography system for detection at 210 nm. The clinical applicability of this assay is demonstrated by the determination of tolazoline serum and urine concentrations in neonates. In addition, the presence of urine conjugates and the extent of serum protein binding were investigated. This assay procedure has the required sensitivity (0.1 microgram/ml), accuracy and precision for both routine monitoring and pharmacokinetic characterization of tolazoline in neonates and adults.
DOI:	10.1016/0378-4347(85)80076-1