Isocratic high-performance liquid chromatographic measurement of optimal 5 alpha-steroid reductase activity in Hep-G2 cells

Measurement of 5 alpha-reductase activity usually involves quantitation of the radiolabelled products of [3H]testosterone. Recently, however, it has been claimed that the activity of 5 alpha-reductase is masked by the activities of 17 beta-hydroxysteroid dehydrogenase and 3-ketosteroid reductase. Th...

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Bibliographic Details

Published in:	Journal of chromatography Vol. 570; no. 2; p. 293
Main Authors:	Stubbs, A P, Murphy, G M, Wilkinson, M L
Format:	Journal Article
Language:	English
Published:	Netherlands 04-10-1991
Subjects:	17-Hydroxysteroid Dehydrogenases - metabolism 3-Oxo-5-alpha-Steroid 4-Dehydrogenase - metabolism Carcinoma, Hepatocellular - enzymology Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Humans Liver Neoplasms - enzymology Testosterone - metabolism Tumor Cells, Cultured
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