Bioaugmentation and Biostimulation of Total Petroleum Hydrocarbon Degradation in a Petroleum-contaminated Soil with Fungi Isolated from Olive Oil Effluent

• Published in: Water, air, and soil pollution Vol. 230; no. 3; pp. 1 - 16
• Main Authors: Essabri, Abduelbaset M. A., Aydinlik, Nur Pasaoglulari, Williams, Ndifreke Etuk
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-03-2019; Springer; Springer Nature B.V
• Subjects: Analysis; Atmospheric Protection/Air Quality Control/Air Pollution; Biodegradable materials; Biodegradation; Bioremediation; Climate Change/Climate Change Impacts; Cluster analysis; Consortia; DNA; Earth and Environmental Science; Environment; Environmental monitoring; Extracellular; Extracellular enzymes; Factor analysis; Fungi; Hydrocarbons; Hydrogeology; Isotopes; Multivariate analysis; Oils & fats; Olive oil; Petroleum; Petroleum hydrocarbons; Soil; Soil contamination; Soil microorganisms; Soil pollution; Soil Science & Conservation; Spacer; Waste management industry; Water Quality/Water Pollution; Weight; Biodegradation; Fungi; Soil; BSM; Petroleum; Contamination
• DOI: 10.1007/s11270-019-4127-8
• EISSN: 1573-2932
• ISSN: 0049-6979

In degradation of total petroleum hydrocarbon, 35 isolates belonging to 11 genera were sanitized and 3 isolates as well as their consortium were initiated to be able to raise in association with petroleum hydrocarbon as sole source of carbon under in vitro circumstances. The isolated strains were grounded on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the utmost potentiality to reduce petroleum hydrocarbon without emerging antagonistic activities were
Aspergillus niger
,
Penicillium ochrochloron
, and
Trichodema viride.
For fungal growth on petroleum hydrocarbon,
P. ochrocholon
gained weight of 44%,
A. niger
49%, and
T. viride
39% within the first 30–40 days. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes, and degraded total petroleum hydrocarbon and
A. niger
strongly degraded total petroleum hydrocarbon with a degradation of about 71.19%. These observations with GC-MS data confirm that these isolates displayed rapid total petroleum hydrocarbon biodegradation within a period of 60 days and the half-life showed that
A. niger
was the shortest with
t
1/2 = 21.280 day
−1
corresponding to the highest percent degradation of 71.19% and first-order kinetic fitted into the present study. By multivariate analysis, five main factors were identified by factor analysis (FA). The first factor (F1) of the fungi species accounts for 20.0% which signifies that fungi species controls the degradation of petroleum variability and hierarchical cluster analysis (HCA) as a dendrogram with five observations and three variables shows two predominant clusters order cluster 1 > 2.