Effect of Excipients on the Stability of Insulin Lispro

• Published in: Macromolecular symposia. Vol. 351; no. 1; pp. 46 - 50
• Main Authors: Misra, Gauri P., Janagam, Dileep R., Lowe, Tao L.
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01-05-2015; Wiley Subscription Services, Inc
• Subjects: chitosan; excipients; Humalog; hyaluronic acid; insulin lispro; Pluronic F-127; stability
• ISSN: 1022-1360; 1521-3900
• DOI: 10.1002/masy.201400215

Summary
The effects of polymers hyaluronic acid (anionic), chitosan (cationic), and Pluronic F‐127 (nonionic) on the stability of the insulin lispro in PBS (pH 7.4) solution at 37°C was investigated using ultra‐performance liquid chromatography. The results were that hyaluronic acid slightly enhanced while chitosan and Pluronic® F‐127 both decreased the stability of insulin lispro. Furthermore, the negative effect of chitosan on insulin lispro's stability was worse than that of Pluronic® F‐127. These results clearly indicate that the macromolecular structure and charge of the polymer excipients had a significant effect on the stability of insulin lispro.