Characterisation of grain quality in diverse sorghum germplasm using a Rapid Visco-Analyzer and near infrared reflectance spectroscopy

• Published in: Journal of the science of food and agriculture Vol. 92; no. 7; pp. 1402 - 1410
• Main Authors: Shewayrga, Hailemichael, Sopade, Peter A, Jordan, David R, Godwin, Ian D
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-05-2012; John Wiley and Sons, Limited
• Subjects: Animals; Carbohydrates; Cluster Analysis; Diet; Dietary Carbohydrates - analysis; Dietary Proteins - analysis; Digestion; Edible Grain; Energy Metabolism; Ethiopia; Feces; Genotype; Grain; grain quality; Humans; ileal digestible energy; Ileum; landraces; NIRS; Principal Component Analysis; Principal components analysis; Seeds - chemistry; Sorghum - chemistry; Sorghum - genetics; Spectroscopy, Near-Infrared - methods; Spectrum analysis; Starch - analysis; Starch - chemistry; Starch - genetics; Swine; Temperature; Viscosity; Ethiopia
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.4714

BACKGROUND: Twenty‐two diverse sorghum landraces, classified as normal and opaque types obtained from Ethiopia, were characterised for grain quality parameters using near infra‐red spectroscopy (NIRS), chemical and Rapid Visco‐Analyzer (RVA) characteristics.
RESULTS: Protein content ranged from 77 to 182 g kg−1, and starch content from 514 to 745 g kg−1. The NIRS analysis indicated the pig faecal digestible energy range from 14.6 to 15.7 MJ kg−1 as fed, and the ileal digestible energy range from 11.3 to 13.9 MJ kg−1 as fed. The normal sorghums had higher digestible energy than the opaque sorghums, which exhibited lower RVA viscosities, and higher pasting temperatures and setback ratios. The RVA parameters were positively correlated with the starch content and negatively correlated with the protein content. The normal and opaque types formed two distinct groups based on principal component and cluster analyses.
CONCLUSION: The landraces were different for the various grain quality parameters with some landraces displaying unique RVA and NIRS profiles. This study will guide utilisation of the sorghum landraces in plant improvement programs, and provides a basis for further studies into how starch and other constituents behave in and affect the properties of these landraces. Copyright © 2011 Society of Chemical Industry