A dynamic and stationary rheological study of erythrocytes incubated in a glucose medium

• Published in: Journal of biochemical and biophysical methods Vol. 62; no. 2; pp. 131 - 141
• Main Authors: Riquelme, Bibiana, Foresto, Patricia, D'Arrigo, Mabel, Valverde, Juana, Rasia, Rodolfo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 28-02-2005
• Subjects: Culture Media; Diabetes; Diabetes Mellitus - blood; Digital image analysis; Dose-Response Relationship, Drug; Edetic Acid - pharmacology; Erythrocyte Deformability - drug effects; Erythrocyte viscoelasticity; Erythrocytes - cytology; Erythrocytes - metabolism; Glucose - metabolism; Glucose - pharmacology; Hemorheology; Humans; Image Processing, Computer-Assisted; Laser diffractometry; Lasers; Non-enzymatic glycosylation; Osmosis; Osmotic Fragility; Rheology; Sodium Chloride - pharmacology; Suspending medium; Temperature; Non-enzymatic glycosylation; Laser diffractometry; Erythrocyte viscoelasticity; Diabetes; Digital image analysis; Suspending medium
• ISSN: 0165-022X; 1872-857X
• DOI: 10.1016/j.jbbm.2004.10.004

A higher than normal glucose concentration in a suspending medium may produce non-enzymatic glycosylation of erythrocyte proteins. This process can modify the viscoelastic properties of erythrocytes. In this paper, we studied the possible relationship between glucose concentration in a suspending medium and erythrocyte rheological parameters. Human venous blood was obtained from the antecubital veins of 10 healthy volunteers. Blood samples were anticoagulated with EDTA and centrifuged. Red blood cells (RBCs) were washed and subsequently divided in aliquots, which were incubated in vitro with glucose solutions of different concentrations. Dynamic and stationary viscoelastic parameters of RBCs were determined by laser diffractometry in an Erythrodeformeter. Aggregate shape parameter (ASP) of the RBCs was determined by digital image processing. Significant changes were observed both in ASP and in rheological parameters when the glucose concentration in the medium was increased, demonstrating that a glucose concentration as low as 1% induces alterations in the mechanical properties of RBCs.