TxBR montage reconstruction for large field electron tomography

• Published in: Journal of structural biology Vol. 180; no. 1; pp. 154 - 164
• Main Authors: Phan, Sébastien, Lawrence, Albert, Molina, Tomas, Lanman, Jason, Berlanga, Monica, Terada, Masako, Kulungowski, Alexander, Obayashi, James, Ellisman, Mark
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2012
• Subjects: 3D reconstruction; Algorithms; Animals; Brain - cytology; Drosophila - cytology; Drosophila - virology; Electron Microscope Tomography - methods; Electron tomography; Imaging, Three-Dimensional - methods; Insect Viruses - physiology; Mice; Montaging; Neurons - ultrastructure; Section flattening; Software; TxBR; Whole cell tomography; Electron tomography; Montaging; 3D reconstruction; TxBR; Whole cell tomography; Section flattening
• ISSN: 1047-8477; 1095-8657
• DOI: 10.1016/j.jsb.2012.06.006

Electron tomography (ET) has been proven an essential technique for imaging the structure of cells beyond the range of the light microscope down to the molecular level. Large-field high-resolution views of biological specimens span more than four orders of magnitude in spatial scale, and, as a consequence, are rather difficult to generate directly. Various techniques have been developed towards generating those views, from increasing the sensor array size to implementing serial sectioning and montaging. Datasets and reconstructions obtained by the latter techniques generate multiple three-dimensional (3D) reconstructions, that need to be combined together to provide all the multiscale information. In this work, we show how to implement montages within TxBR, a tomographic reconstruction software package. This work involves some new application of mathematical concepts related to volume preserving transformations and issues of gauge ambiguity, which are essential problems arising from the nature of the observation in an electron microscope. The purpose of TxBR is to handle those issues as generally as possible in order to correct for most distortions in the 3D reconstructions and allow for a seamless recombination of ET montages.