A novel cadmium induced protein in wheat: characterization and localization in root tissue

• Published in: Biologia plantarum Vol. 52; no. 2; pp. 343 - 346
• Main Authors: Mittra, B.,Fakir Mohan Univ., Balasore (India). Dept. of Biosciences and Biotechnology, Sharma, S.,All India Inst. of Medical Sciences, New Delhi (India). Dept. of Biophysics, Das, A.B.,Regional Plant Resource Center, Bhubaneswar (India). Cytogenetics Lab, Henry, S.L.,University of Glasgow (United Kingdom). Dept. of Plant Sciences, Das, T.K.,All India Inst. of Medical Sciences, New Delhi (India). Electron Microscopic Facility, Ghosh, P.,University of Delhi, New Delhi (India). Dept. of Biophysics, Ghosh, S.,University of Delhi, New Delhi (India). Dept. of Biophysics, Mohanty, P.,Regional Plant Resource Center, Bhubaneswar (India). Cytogenetics Lab
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-06-2008
• Subjects: Biomedical and Life Sciences; BLE; Brief Communication; CADMIO; CADMIUM; ESTRES; http://www.fao.org/aos/agrovoc#c_1178; http://www.fao.org/aos/agrovoc#c_4810; http://www.fao.org/aos/agrovoc#c_6259; http://www.fao.org/aos/agrovoc#c_6651; http://www.fao.org/aos/agrovoc#c_6945; http://www.fao.org/aos/agrovoc#c_7452; http://www.fao.org/aos/agrovoc#c_7951; http://www.fao.org/aos/agrovoc#c_8045; http://www.fao.org/aos/agrovoc#c_8373; Life Sciences; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; Plant Sciences; PLANTULAS; PLANTULE; PROTEINAS; PROTEINE; PROTEINS; RACINE; RAICES; ROOTS; SEEDLINGS; STRESS; TRIGO; TRITICUM AESTIVUM; ULTAESTRUCTURA; ULTRASTRUCTURE; WHEATS; cadmium stress; N-terminal sequencing; stress protein; immunolocalization; Triticum aestivum
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-008-0070-z

A 51-kDa soluble protein was over-expressed in Triticum aestivum seedlings by the treatment of seeds before germination with 50 microM CdCl2 for 48 h and subsequently washed off Cd2+. This protein, designated as Cd stress associated protein (CSAP), was purified. Polyclonal antibody was raised against CSAP for localizing the protein in root tissue of treated and control seedlings. It was observed that CSAP was located below the plasma membrane and outer periphery of the tonoplast. This unique type of organized localization of CSAP is suggestive of defensive role against metal phytotoxicity. N-terminal analysis of CSAP and expressed sequence tags (EST) database search of wheat sequences suggests that this protein has not been reported earlier in higher plants.