Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells

• Published in: Molecular & cellular toxicology Vol. 16; no. 3; pp. 321 - 330
• Main Authors: Adriana-Berenice, Peralta-Vega, Alberto, Parra-Barrera, del Pilar, Ramos-Godínez María, Rebeca, López-Marure, José, Arellano-Galindo, Gutiérrez-Iglesias, Gisela
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01-07-2020; Springer Nature B.V; 대한독성 유전단백체 학회
• Subjects: Biomedical and Life Sciences; Cell Biology; Cell proliferation; Cosmetics; Cytosol; Life Sciences; Mesenchymal stem cells; Nanoparticles; Original Article; Osteoblastogenesis; Pharmacology/Toxicology; Stem cells; Titanium dioxide; Viability; 생물학; Mesenchymal stem cell; Nanoparticle; Toxicity; Adipoblasts; Cell differentiation; Titanium dioxide; Viability; Osteoblasts
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-020-00084-8

Background
Titanium dioxide (TiO
2
) is employed in the production of cosmetics, sunscreen, food, and drugs; however, TiO
2
is toxic at the nanometric scale.
Objective
To analyze the in vitro toxic effect of TiO
2
NPs on mesenchymal stem cells (hMSCs).
Methods
The hMSCs from Wharton`s jelly were exposed to 4, 62.5 and 500 μg/mL of TiO
2
NPs. Viability and cell proliferation tests were carried out at 2, 5 and 7 days of exposure. Additionally, the osteogenic and adipogenic differentiation capacity was evaluated.
Results
TiO
2
NPs had a perinuclear arrangement and internalized in vesicles into cytosol, diminished significantly the viability (40% and 30% and 30%) and the proliferation cellular (35%, 50% and 55%), differentiation to osteoblast (38%) and adipoblasts (20%).
Conclusion
TiO
2
NPs affect viability, proliferation, and differentiation of hMSCs. Our result suggests more regulation of the use of TiO
2.