Quasichemical description of the cell death kinetics of cellular biosensor Spirostomum ambigua for testing the biological activity of aqueous solutions

• Published in: Journal of water chemistry and technology Vol. 39; no. 2; pp. 97 - 102
• Main Authors: Goncharuk, V. V., Syroeshkin, A. V., Zlatskiy, I. A., Uspenskaya, E. V., Orekhova, A. V., Levitskaya, O. V., Dobrovolskiy, V. I., Pleteneva, T. V.
• Format: Journal Article
• Language: English
• Published: New York Allerton Press 01-03-2017; Springer Nature B.V
• Subjects: Activation energy; Aquatic Pollution; Aqueous solutions; Biological activity; Biological Methods of Water Treatment; Biosensors; Biotesting; Cell death; Cells; Drugs; Earth and Environmental Science; Energy; Environment; Industrial Chemistry/Chemical Engineering; Kinetics; Laboratory animals; Laboratory tests; Ligands; Mortality; Oral administration; Solutions; Temperature effects; Testing; Toxicants; Waste Water Technology; Water Industry/Water Technologies; Water Management; Water Pollution Control; Water Quality/Water Pollution; toxicity; activation energy; kinetic isotope effect; biosensor; biotesting of water
• ISSN: 1063-455X; 1934-936X
• DOI: 10.3103/S1063455X17020072

A kinetic method of biotesting of aqueous solutions using isolated cells of unicellular organism
Spirostomum ambigua
has been described. A kinetic scheme of interaction of cellular biosensor with ligands based on the Michaelis-Menten mechanism was proposed. It was shown that the death rate of
S. ambigua
featured the Arrhenius-type dependence on temperature. The activation energy was determined for different toxicants and drugs. For many compounds the activation energy of slow stage of the process of cellular biosensor death linearly correlates with parameter DL
50
for the same substances at their oral administration to laboratory animals.