Deletion of AhR attenuates fear memory leaving other types of memory intact

• Published in: Behavioural brain research Vol. 451; p. 114505
• Main Authors: Sun, Na, Wang, Hao, Wang, Xin-Yue, Yu, Qi, Han, Jin-Yuan, Huang, Yan, Zhou, Wen‑Xia
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05-08-2023
• Subjects: Animals; Anxiety - genetics; Aryl hydrocarbon receptor; Emotional response; Fear - physiology; Fear memory; Memory Disorders; Mice; Mice, Inbred C57BL; Mice, Knockout; Receptors, Aryl Hydrocarbon; Stress; CFC; EPM; ANS; CNS; HR; AhR-KO; HRV; Fear memory; PNS; OFT; ASR; MWM; SBT; bHLH-PAS; NORT; ECG; HST; AhR; Stress; Emotional response; PTSD; Aryl hydrocarbon receptor; HPA; LF; HF
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2023.114505

The aryl hydrocarbon receptor (AhR), a classic “environmental sensor”, has been found to play an important role in cognitive and emotional function. Recent studies showed AhR deletion led to an attenuated fear memory, providing a potential target against fear memory, whether it is the consequence of attenuated sense of fear or memory ability deficit or both is unclear. Here this study aims to work this out. The freezing time in contextual fear conditioning (CFC) reduced significantly in AhR knockout mice, indicating an attenuated fear memory. Hot plate test and acoustic startle reflex showed that AhR knockout did not change the pain threshold and hearing, excluded the possibility of sensory impairments. Results from NORT, MWM and SBT showed that deletion of AhR had little effects on other types of memory. But the anxiety-like behaviors reduced both in naïve or suffered (tested after CFC) AhR knockout mice, showing that AhR-deficient mice have a reduced basal and stressful emotional response. The basal low-frequency to high-frequency (LF/HF) ratio of the AhR knockout mice was significantly lower than that of the control group, indicating lower sympathetic excitability in the basal state, suggesting a low level of basal stress in the knockout mice. Before and after CFC, the LF/HF ratio of AhR-KO mice tended to be significantly lower than that of WT mice, and their heart rate was significantly lower; and the AhR-KO mice also has a decreased serum corticosterone level after CFC, suggesting a reduced stress response in AhR knockout mice. Altogether, the basal stress level and stress response were significant reduced in AhR knockout mice, which might contribute to the attenuated fear memory with little impairment on other types of memory, suggesting AhR as a “psychologic sensor” additional to “environmental sensor”.
•AhR is involved in the process of fear memory.•AhR-deficient mice have a reduced emotional response.•Deletion of AhR attenuates fear memory by reducing stress response.