Applicability of the critical-chromatography concept to proteomics problems: Separation of peptides modeled by a heterogeneous rod

• Published in: Polymer science. Series A, Chemistry, physics Vol. 59; no. 3; pp. 451 - 464
• Main Authors: Gorshkov, A. V., Goloborodko, A. A., Pridatchenko, M. L., Tarasova, I. A., Rozdina, I. G., Evreinov, V. V., Gorshkov, M. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-05-2017
• Subjects: Chemistry; Chemistry and Materials Science; Polymer Sciences; Theory and Simulation
• ISSN: 0965-545X; 1555-6107
• DOI: 10.1134/S0965545X17030063

The problems of separation of short peptides are considered in terms of the model of rigid rod adsorption. Analytical expressions relating the retention volume to the sequence of amino-acid residues in the gradient elution are obtained. The model of adsorption of a peptide as a rigid rod is compared with the model of its adsorption as a random-walk chain. The transition of rodlike peptides to the adsorbed state is more abrupt compared with the random-walk chain having the same sequence, while with an increase in the peptide length the random-walk chain model becomes more accurate. It is shown that the model of adsorption of a peptide as a rigid rod for short peptides fits the experimental data and correctly predicts the character of separation of peptides having equal lengths and identical amino-acid compositions but slightly differing in the alternation of residues in a chain.