Incorporation of new neurons in the olfactory bulb after paced mating in the female rat

• Published in: Behavioural brain research Vol. 343; pp. 95 - 101
• Main Authors: Alvarado-Martínez, R., Paredes, R.G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 02-05-2018
• Subjects: Accessory olfactory bulb (AOB); Animals; Antigens, Nuclear - metabolism; Bromodeoxyuridine; Cell Count; Female; Fluorescent Antibody Technique; Main olfactory bulb (MOB); Nerve Tissue Proteins - metabolism; Neurogenesis; Neurogenesis - physiology; Neurons - cytology; Neurons - physiology; Non-paced mating (NPM); Olfactory Bulb - cytology; Olfactory Bulb - physiology; Ovariectomy; Paced mating (PM); Rats, Wistar; Sexual Behavior, Animal - physiology; Non-paced mating (NPM); Paced mating (PM); Accessory olfactory bulb (AOB); Neurogenesis; Main olfactory bulb (MOB)
• ISSN: 0166-4328; 1872-7549
• DOI: 10.1016/j.bbr.2018.02.006

•Repeated paced mating increases the number of cells in the accessory olfactory bulb.•Repeated paced mating increases the number of neurons in the main olfactory bulb.•Repeated mating contributes to integration of new cells and neurons.
One of the regions that constantly produces neurogenesis in the adult brain is the subventricular zone (SVZ), whose new cells migrate to the olfactory bulbs (OB). When the females regulate the copulatory events (paced mating) the number of new cells in the SVZ increases, as well as those observed in the OB 15 days later. However, no changes were observed in the number of cells 45 days after the females paced the sexual interaction. Constant sensory stimulation is an important promoter of cell survival in the OB circuit. Hence, we increased the number of mating sessions in this study to cover the period where stimulation of the new cells is critical for their incorporation into pre-existing circuits in the OB. Ovariectomized female Wistar rats, were injected with the mitotic marker 5-bromo-2′-deoxyuridine (BrdU, 100 mg/kg, per injection) before, at the end and one hour after mating. Sexual behavior was recorded for 1 h in 10 weekly sessions. After the last mating session, brain sections were processed to determine BrdU immunoreactivity. Our results indicate that females that paced the sexual interaction for 10 sessions had a higher number of cells in the glomerular layer (GL) of the accessory olfactory bulb (AOB) and a higher number of neurons in the granular layer (GrL) of the main olfactory bulb (MOB) in comparison to the control group. These results indicate that continued sexual interaction contributes to the integration of new cells and neurons, induced in the first sexual experience, into pre-exiting circuits of the OB.