Quantification and evaluation of kinetic bio-catalytic pathway of horseradish peroxidase in an electron mediated reaction system and its applications in plant extracts

[Display omitted] ► 2,5-Dimethoxyaniline is used as a chromogenic co-substrate for peroxidase assay. ► A mathematical model has been proposed for the evaluation of catalytic parameters. ► Peroxidase activity in some of crude medicinal plant species has been assessed. ► Peroxidase activity has been r...

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Bibliographic Details
Published in:	Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 102; pp. 75 - 81
Main Authors:	Krishna, Honnur, Nagaraja, Padmarajaiah, Shivakumar, Anantharaman, Chamaraja, Nelligere A., Aradhana, Narayan
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 01-02-2013
Subjects:	2,5-Dimethoxyaniline Aniline Compounds - metabolism Armoracia - enzymology Bio-catalyst Crude plant extracts Electrons Enzyme Assays - methods Horseradish Peroxidase - metabolism Horseradish peroxidase assay Hydrogen Peroxide - analysis Hydrogen Peroxide - metabolism Kinetics Mathematical model Mediated electron transfer system Models, Biological Peroxidase - metabolism Plant Extracts - metabolism Plants, Medicinal - enzymology Plants, Medicinal - metabolism Spectrophotometry - methods 2,5-Dimethoxyaniline [H2O2]o HRP [D]o KeffD and KeffG KmH2O2 VmaxH2O2 Bio-catalyst KpowD and KpowG DMA Vo 2,4-DMA Horseradish peroxidase assay Mathematical model Crude plant extracts Mediated electron transfer system
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