Heterogeneous Macromolecular crowding effect on nucleation-independent fibril formation of Lysozyme: Spectroscopic analysis of Structure, Stability, and fibrillation rate
[Display omitted] •Studied effect of homotypic and heterotypic crowding on fibril rate and stability.•In mixed crowders fibril rate is comparable or slower than the individual crowders.•Crowders stabilize lysozyme and induce two-state unfolding at higher concentration.•Stability or fibril rate is no...
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| Published in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 315; p. 124276 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier B.V
05-07-2024
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| Online Access: | Get full text |
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| Summary: | [Display omitted]
•Studied effect of homotypic and heterotypic crowding on fibril rate and stability.•In mixed crowders fibril rate is comparable or slower than the individual crowders.•Crowders stabilize lysozyme and induce two-state unfolding at higher concentration.•Stability or fibril rate is not correlated with size or viscosity of the crowders.•Excluded volume and direct interactions contribute to changes induced by crowders. |
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| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2024.124276 |