Human‐ and mouse‐derived neurons can be simultaneously obtained by co‐cultures of human oral mucosal stem cells and mouse neural stem cells

• Published in: Oral diseases Vol. 24; no. 1-2; pp. 5 - 10
• Main Authors: Alajbeg, I, Alić, I, Andabak‐Rogulj, A, Brailo, V, Mitrečić, D
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01-03-2018
• Subjects: Animals; Astrocytes; Cell Communication; Cell culture; Cell Differentiation; Cell Survival; Coculture Techniques; Culture Media; Dentistry; differentiation; Humans; Ischemia; Mice; mouse neural stem cells; Mouth Mucosa - cytology; Mucosa; neural crest stem cells; Neural stem cells; Neural Stem Cells - cytology; Neural Stem Cells - physiology; neuroectoderm; Neurons; Oct-4 protein; Oral diseases; oral mucosa; Stem cell transplantation; Stem cells; Tubulin; mouse neural stem cells; neural crest stem cells; differentiation; oral mucosa; neuroectoderm
• ISSN: 1354-523X; 1601-0825
• DOI: 10.1111/odi.12776

Objective
To observe simultaneous differentiation and analyse possible interactions between co‐cultured human oral mucosal stem cells (hOMSC) and mouse neural stem cells (mNSC).
Materials and Methods
hOMSC and mNSC were co‐cultured in mouse and in human medium, and their immunocytochemical characterization to detect survival rate and differentiation pattern was performed. Co‐cultures in different media were compared to hOMSC in human medium and mNSC in mouse medium as controls.
Results
Co‐culture of hOMSC and mNSC in medium for human cells led to normal differentiation pattern of human cells, while mNSC were directed towards astrocytes. When the same cells were cultivated in the mouse medium, both cell types succeeded to form neurons, although mNSC showed a tendency to overgrow hOMSC. hOMSC alone in the human‐specific medium differentiated towards ectodermal (Oct4, Map2) and mesodermal (Osterix) cell populations. mNSC in the mouse‐specific medium differentiated towards Map2‐, β3‐tubulin‐ and NeuN‐positive neurons.
Conclusions
hOMSC and mNSC can form co‐cultures. Different media considerably affected the differentiation pattern of co‐cultures, whereas one cell population itself modestly influenced differentiation of the other cell type. The in vitro differentiation pattern of hOMSC in the mouse neural tissue environment suggested that hOMSC could be beneficial in the brain tissue affected by ischaemia.