The effect of light regime and time of slaughter in broiler on broiler performance, liver antioxidant status, and expression of genes related to peptide absorption in the jejunum and melatonin synthesis in the brain

• Published in: Journal of animal physiology and animal nutrition Vol. 107; no. 2; pp. 607 - 620
• Main Authors: Pontes, Mauricio Pires de, Khatlab, Angélica de Souza, Del Vesco, Ana Paula, Granzoto, Gabriela Hernandes, Soares, Maria Amélia Menck, Sousa, Fabiana Cristina Belchior de, Souza, Maria Luiza Rodrigues de, Gasparino, Eliane
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01-03-2023
• Subjects: Animals; Antioxidants; Aralkylamine N-acetyltransferase; artificial lighting; arylalkylamine N‐acetyltransferase; Birds; Brain; Brain - metabolism; Catalase; Catalase - metabolism; Chickens - metabolism; Circadian Rhythm; Gene expression; Jejunum; Jejunum - metabolism; Light; Lipid peroxidation; Lipids; Liver; Liver - metabolism; Melatonin - metabolism; Nitrites; Oxidants; Oxidation; Oxidizing agents; Peptide transporter; peptide transporter 1; Peptides; Proteins; redox; Slaughter; Small intestine; Superoxide dismutase; Superoxide Dismutase - metabolism; artificial lighting; peptide transporter 1; arylalkylamine N-acetyltransferase; redox
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.13712

This study aimed to assess the effects of light regime and time of slaughter on primal cut and organ weights, peptide transporter 1 (PEPT1) gene expression in the jejunum, arylalkylamine N‐acetyltransferase (AANAT) gene expression in the brain, and liver oxidant/antioxidant status in broilers aged 37 days. The experiment was conducted in a factorial completely randomized design, with two light regimes (intermittent light varying according to bird age and continuous light under an 18 h light/6 h dark photoperiod) and four times of slaughter (2:00, 8:00, 14:00 and 20:00 h). There was an interaction effect on PEPT1 and AANAT expression, lipid and protein oxidation and superoxide dismutase (SOD) activity. In both light regimes, PEPT1 expression responded cubically to slaughter time. In the continuous light group, PEPT1 expression was highest in birds slaughtered at 2:00 and 14:00 h, whereas, in the intermittent light treatment, expression was highest at 8:00 h. In the continuous light regime, AANAT expression had a cubic relationship with time of slaughter, with the greatest values recorded at 20:00 h. In the intermittent light regime, slaughter time showed a cubic effect on lipid oxidation, which was highest at 8:00 h. In the continuous light group, there was a cubic effect on nitrite concentration, lipid oxidation, protein oxidation, and SOD activity; nitrite levels, lipid oxidation, and protein oxidation were highest and SOD activity was lowest in birds slaughtered at 14:00 h. Time of slaughter influenced catalase activity, which responded cubically; catalase activity was lowest at 8:00 and 14:00 h. This study is the first to demonstrate that PEPT1 expression in the jejunum of broilers follows a diurnal rhythm and varies according to light regime. The results also suggest that mainly continuous lighting and slaughter at 14:00 h when the animals are possibly more active may be more stressful to broilers.