Exosome‐derived differentiation antagonizing non‐protein coding RNA with risk of hepatitis C virus‐related hepatocellular carcinoma recurrence

• Published in: Liver international Vol. 41; no. 5; pp. 956 - 968
• Main Authors: Wang, Shu‐Chi, Li, Chia‐Yang, Chang, Wen‐Tsan, Cheng, Wei‐Chung, Yen, Chia‐Hung, Tu, Wen‐Yu, Lin, Zu‐Yau, Lin, Ching‐Chih, Yeh, Ming‐Lun, Huang, Chung‐Feng, Huang, Jee‐Fu, Dai, Chia‐Yen, Chuang, Wan‐Long, Chen, Yao‐Li, Yu, Ming‐Lung
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-05-2021
• Subjects: Biomarkers; Confidence intervals; Differentiation; differentiation antagonizing non‐protein coding RNA; Exosomes; Gene sequencing; HCC; Hepatitis; Hepatitis C; hepatitis C virus; Hepatocellular carcinoma; Interferon; Liver cancer; Neoplasia; Neoplasms; Non-coding RNA; Polymerase chain reaction; Proteins; recurrence; Ribonucleic acid; RNA; RNA viruses; Target recognition; Therapeutic targets; Tissues; Tumors; Viruses; recurrence; hepatitis C virus; differentiation antagonizing non-protein coding RNA; HCC; exosomes
• ISSN: 1478-3223; 1478-3231
• DOI: 10.1111/liv.14772

Background & Aims
Differentiation antagonizing non‐protein coding RNA is associated with various types of neoplasms. Hepatitis C virus‐related hepatocellular carcinoma has a high risk of recurrence. Here we determined the role of differentiation antagonizing non‐protein coding RNA in hepatitis C virus‐related hepatocarcinogenesis and identified potential therapeutic targets and non‐invasive prognostic markers for long‐term outcome of hepatitis C virus‐related hepatocellular carcinoma after surgical resection.
Methods
Differentiation antagonizing non‐protein coding RNAs relevant to hepatitis C virus‐related hepatocellular carcinoma were identified through comparative RNA‐sequencing of tumour and adjacent non‐tumour (ANT) tissues in a screening set, and were validated using real‐time polymerase chain reaction. Target long non‐coding RNAs (lncRNAs) in tissues and serum exosomes were used to predict the recurrence of hepatitis C virus‐related hepatocellular carcinoma after curative surgical resection in a large application cohort from 2005 to 2012.
Results
We confirmed that differentiation antagonizing non‐protein coding RNA was upregulated following hepatitis C virus infection and identified as the lncRNA most relevant to hepatitis C virus‐related hepatocellular carcinoma in tumour tissues as compared to that in ANT tissues. In 183 hepatitis C virus‐related hepatocellular carcinoma patients followed for 10 years after curative HCC resection, the expression level of circulating exosomal differentiation antagonizing non‐protein coding RNA was positively associated with HCC recurrence and was the most predictive factor associated with HCC recurrence and mortality (hazard ratio/95% confidence intervals: 7.0/4.3‐11.6 and 2.7/1.5‐5.1 respectively).
Conclusions
Differentiation antagonizing non‐protein coding RNA is highly relevant to disease progression of hepatitis C virus‐related hepatocellular carcinoma. Our finding indicated that circulating exosomal differentiation antagonizing non‐protein coding RNA might serve as a non‐invasive prognostic biomarker for hepatitis C virus‐related hepatocellular carcinoma.