The dietary isoflavone daidzein mitigates oxidative stress, apoptosis, and inflammation in CDDP‐induced kidney injury in rats: Impact of the MAPK signaling pathway

• Published in: Journal of biochemical and molecular toxicology Vol. 34; no. 2; pp. e22431 - n/a
• Main Authors: Tomar, Ameesha, Kaushik, Swati, Khan, Sana Irfan, Bisht, Khushboo, Nag, Tapas Chandra, Arya, Dharamvir Singh, Bhatia, Jagriti
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-02-2020
• Subjects: Acute Kidney Injury - chemically induced; Acute Kidney Injury - diet therapy; Animals; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antioxidants; Antioxidants - administration & dosage; Antioxidants - pharmacology; Antioxidants - therapeutic use; Apoptosis; Apoptosis - drug effects; Chemotherapy; Cisplatin; Cisplatin - adverse effects; Cisplatin - pharmacology; Cytokines - blood; Daidzein; Diet; Inflammation; Isoflavones; Isoflavones - administration & dosage; Isoflavones - pharmacology; Isoflavones - therapeutic use; Kidney - drug effects; Kidneys; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver - drug effects; Male; MAP kinase; MAP Kinase Signaling System - drug effects; Nephritis - diet therapy; nephrotoxicity; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Rats; Renal function; Signal transduction; Signaling; Toxicity; Treatment Outcome; apoptosis; Daidzein; nephrotoxicity; cisplatin; inflammation; oxidative stress
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.22431

Cisplatin‐induced nephrotoxicity persists as a clinical problem despite several supportive measures to alleviate renal damage. Daidzein (DZ), a dietary isoflavone having antioxidant and anti‐inflammatory activity, is investigated in this study for protective effects against cisplatin‐induced renal injury in rats. DZ (25, 50, or 100 mg/kg; intraperitoneally; 10 days) was administered along with Cisplatin, single dose, on the 7th day of the experiment. On the 11th day, the rats were euthanized, and different samples were collected for analysis. Biochemical, histopathological, and molecular parameters were assessed to evaluate the effect of daidzein. Cisplatin injection resulted in renal dysfunction, lipid peroxidation that led to consumption of antioxidants, exaggerated apoptosis, and inflammation. These changes were associated with increase in the signaling proteins. DZ attenuated the toxic effects of cisplatin on the kidney at 100 mg/kg dose. The study concludes with the finding that daidzein imparts protection against the nephrotoxic effect of Cisplatin and can be considered as a novel, potential therapy.