Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice

• Published in: Journal of cellular biochemistry Vol. 118; no. 4; pp. 709 - 717
• Main Authors: Zarzecki, Micheli Stéfani, Bortolotto, Vandreza Cardoso, Poetini, Márcia Rósula, Araujo, Stífani Machado, de Paula, Mariane Trindade, Roman, Silvane Souza, Spiazzi, Cristiano, Cibin, Franciele Weber Santos, Rodrigues, Oscar Endrigo Dorneles, Jesse, Cristiano Ricardo, Prigol, Marina
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-04-2017
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Antioxidants - pharmacology; Cytokines - biosynthesis; Disease Models, Animal; Female; IBD; INFLAMMATION; Inflammation Mediators - metabolism; Inflammatory Bowel Diseases - drug therapy; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; KEYWORDS; Mice; Organoselenium Compounds - pharmacology; OXIDATIVE STRESS; Oxidative Stress - drug effects; Peroxidase - metabolism; SELENIUM; Trinitrobenzenesulfonic Acid - toxicity; OXIDATIVE STRESS; IBD; INFLAMMATION; KEYWORDS; SELENIUM
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.25670

ABSTRACT
This study aims to investigate the protective effect of p‐chloro‐phenyl‐selenoesterol [PCS; 0,2 mg/kg; 10 ml/kg i.g.) in colitis induced by 2,4,6‐trinitrobenzene sulfonic acid [TNBS; 2 mg/100 µl 50% ethanol; intrarectally) in mice. Several parameters including weight, length, histological analyses determination, thiobarbituric acid reactive species, reactive species levels, superoxide dismutase, catalase, and myeloperoxidase (MPO) activity of colon were evaluated. The serum levels of tumor necrosis factor alpha [TNF‐α) and interleukin 6 [IL‐6) were also assessed. Treatment with PCS reduced the clinical and histopathologic severity of TNBS‐induced colitis, characterized by colon length reduction and increased colon weight and microscopic intestinal inflammation. The therapeutic effects of PCS in this model were associated with significant decrease in proinflammatory cytokines TNF‐α and IL‐6 and decrease in MPO activity. Furthermore, combined with improvements in inflammatory parameters, treatment with the PCS was able to decrease oxidative stress and to prevent the decrease in antioxidant defenses in animals with TNBS‐induced colitis. This finding suggests that PCS can improve experimental colitis in mice and it could be a potential therapeutic agent for the treatment of patients with IBD. J. Cell. Biochem. 118: 709–717, 2017. © 2016 Wiley Periodicals, Inc.
The main effects of p‐chloro‐phenyl‐selenoesterol on TNBS‐induced inflammatory bowel disease in mice.