DNA replication and sister chromatid cohesion 1 promotes breast carcinoma progression by modulating the Wnt/β-catenin signaling and p53 protein

• Published in: Journal of biosciences Vol. 45; no. 1
• Main Authors: Jin, Guangchao, Wang, Wensheng, Cheng, Peng, Tian, Yunqi, Zhang, Luxiao, Niu, Hu
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-12-2020; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Breast cancer; Breast carcinoma; Cell Biology; Cell migration; Cell proliferation; Cyclin D1; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA replication; Gene set enrichment analysis; Life Sciences; Medical prognosis; Microbiology; mRNA; Neoplasms; Nucleotide sequence; p53 Protein; PCR; Plant Sciences; Proliferation; Proteins; Signaling; Survival; Tumor cells; Wnt protein; Zoology; β-Catenin; migration and invasion; Cell viability; GSK-3β/β-catenin; DSCC1
• ISSN: 0250-5991; 0973-7138
• DOI: 10.1007/s12038-020-00100-y

The objective of this study is to assess the prognostic and functional role of DSCC1 in breast carcinoma, as well as the potential mechanism. Based upon the TCGA data, the expression pattern and prognostic value of DSCC1 in breast carcinoma was evaluated. The mRNA and protein levels of molecules were determined using qRT-PCR and Western blot. In vitro functional role of DSCC1 in tumor cells was determined using cell counting kit 8, clone formation, and Transwell assays. Gene set enrichment analysis (GSEA) was conducted to determine DSCC1 related gene sets, which are further confirmed by Western blot. The results showed that DSCC1 is overexpressed in breast carcinoma tissues and its high expression was linked to shorter overall survival. Overexpression of DSCC1 facilitated the proliferation, invasion and migration of breast carcinoma cells, while knockdown of DSCC1 showed opposite outcomes. GSEA showed that high DSCC1 expression had a positive correlation with p53, and Wnt signaling-related molecules. Western blot showed that silencing DSCC1 increased the levels of p53 and p-β-catenin, whereas decreased p-GSK-3β and cyclin D1 expression. These observations illustrate that DSCC1 emerges a well value on the diagnosis and prognosis of breast carcinoma, and facilitates the progression of breast carcinoma partly by activating Wnt/β-catenin signaling and inhibiting p53.