Self-Assembly Stereo-Specific Synthesis of Silver Phosphate Microparticles on Bacterial Cellulose Membrane Surface For Antimicrobial Applications

• Published in: Colloid and interface science communications Vol. 26; pp. 7 - 13
• Main Authors: Bayón, Bernardo, Cacicedo, Maximiliano L., Álvarez, Vera A., Castro, Guillermo R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-09-2018
• Subjects: Antimicrobial activity; Bacterial cellulose; Ciprofloxacin; Drug delivery; Hybrid material; Silver phosphate microparticles; Bacterial cellulose; Antimicrobial activity; Drug delivery; Ciprofloxacin; Silver phosphate microparticles; Hybrid material
• ISSN: 2215-0382; 2215-0382
• DOI: 10.1016/j.colcom.2018.07.002

In situ self-assembly stereo-specific synthesis of silver phosphate (AgP) microparticles (MPs) in one side bacterial cellulose (BC) membrane surface and loading of ciprofloxacin provides BC scaffolds with a broad-spectrum antimicrobial activity against Escherichia coli and Staphylococcus aureus. The BC membranes containing AgP-MPs can be loaded >5 times with ciprofloxacin compared to naked BC. The reuse of ciprofloxacin-loaded AgP-BC scaffolds in fresh cultures of both tested microbes showed high inhibition haloes without any signal of microbial resistance or scaffold activity decay after 30 days of assays. Additionally, microbial biofilms of E. coli and S. aureus were mostly killed by AgP-BC scaffolds loaded with ciprofloxacin in 1 h. The biophysical characterization of the hybrid AgP-BC scaffolds was performed by scanning electron microscopy (SEM), vibrational spectroscopy (FTIR), calorimetric techniques and X-ray diffraction (XRD). SEM images display AgP-MP spheroid structures with a high homogenous distribution on BC scaffold surfaces. The properties of the novel AgP-BC matrices are very promissory for biomedical applications, like a therapeutic dressing patches for the prevention and treatment of microbial infections in skin wounds and burns.
Graphical Abstract [Display omitted]
•Silver phosphate microparticles (AgP-MPs) were synthesized stereo specifically on one side of the bacterial cellulose.•The load of ciprofloxacin is 5 times higher in AgP-BC matrices rather than native BC membranes.•Ciprofloxacin can be released from AgP-BC membranes in a controlled manner.•The AgP-BC scaffold containing ciprofloxacin showed high antimicrobial activity against Gram(+) and Gram(−) microorganisms.•The hybrid AgP-BC scaffold has a residual antimicrobial effect for at least one month.