Characterization of Arsenic-Resistant Klebsiella pneumoniae RnASA11 from Contaminated Soil and Water Samples and Its Bioremediation Potential

• Published in: Current microbiology Vol. 78; no. 8; pp. 3258 - 3267
• Main Authors: Kumar, Prahalad, Dash, Biplab, Suyal, Deep Chandra, Gupta, S. B., Singh, Anup Kumar, Chowdhury, Tapas, Soni, Ravindra
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-08-2021; Springer Nature B.V
• Subjects: Arsenate reductase; Arsenates; Arsenic; Arsenite; Atomic absorption analysis; Atomic absorption spectroscopy; Bacteria; Biomedical and Life Sciences; Bioremediation; Biotechnology; Contamination; Deoxyribonucleic acid; DNA; Efflux; Gene pool; Heavy metals; Industrial pollution; Intensive farming; Klebsiella; Klebsiella pneumoniae; Life Sciences; Microbiology; Reductases; rRNA 16S; Soil bacteria; Soil contamination; Soil microorganisms; Soil pollution; Soil resistance; Soil water; Soils; Spectral analysis; Water analysis; Water pollution; Water sampling
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-021-02602-w

Rapid industrialization and intensive agriculture activities have led to a rise in heavy metal contamination all over the world. Chhattisgarh (India) being an industrial state, the soil and water are thickly contaminated with heavy metals, especially from arsenic (As). In the present study, we isolated 108 arsenic-resistant bacteria (both from soil and water) from different arsenic-contaminated industrial and mining sites of Chhattisgarh to explore the bacterial gene pool. Further, we screened 24 potential isolates out of 108 for their ability to tolerate a high level of arsenic. The sequencing of the 16S rRNA gene of bacterial isolates revealed that all these samples belong to different diverse genera including
Bacillus, Enterobacter
,
Klebsiella
,
Pantoea
,
Acinetobacter
,
Cronobacter, Pseudomonas
and
Agrobacterium
. The metal tolerance ability was determined by amplification of arsB (arsenite efflux gene) and arsC (arsenate reductase gene) from chromosomal DNA of isolated RnASA11, which was identified as
Klebsiella pneumoniae
through in silico analysis. The bacterial strains RpSWA2 and RnASA11 were found to tolerate 600 mM As (V) and 30 mM As (III) but the growth of strain RpSWA2 was slower than RnASA11. Furthermore, atomic absorption spectroscopy (AAS) of the sample obtained from bioremediation assay revealed that
Klebsiella pneumoniae
RnASA11 was able to reduce the arsenic concentration significantly in the presence of arsenate (44%) and arsenite (38.8%) as compared to control.