Investigation of the probiotic and metabolic potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from apiaries

• Published in: Archives of microbiology Vol. 204; no. 7; p. 432
• Main Authors: Simsek, Duygu, Kiymaci, Merve Eylul, Tok, Kenan Can, Gumustas, Mehmet, Altanlar, Nurten
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-07-2022; Springer Nature B.V
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Apilactobacillus; Bacteria; Bees; Biochemistry; Biomedical and Life Sciences; Biosensors; Biotechnology; Cell Biology; Clindamycin; Consumer products; Diacetyl; Ecology; Enterococcus faecalis; Erythromycin; Evaluation; Fructobacillus tropaeoli; Fructose; Kanamycin; Lactic acid; Lactic acid bacteria; Life Sciences; Metabolism; Microbial Ecology; Microbiology; Microbiota; Original Paper; Pancreatin; Pepsin; pH effects; Probiotics; Quorum sensing; rRNA 16S; Streptomycin; Fructophilic bacteria; Antimicrobial resistance; Anti-quorum sensing; One Health
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-03000-x

Honeybee products have been among important consumer products throughout history. Microbiota has attracted attention in recent years due to both their probiotic value and industrial potential. Fructophilic lactic acid bacteria (FLAB), whose field of study has been expanding rapidly in the last 20 years, are among the groups that can be isolated from the bee gut. This study aimed to isolate FLAB from the honeybees of two different geographic regions in Turkey and investigate their probiotic, metabolic and anti-quorum sensing (anti-QS) potential. Metabolic properties were investigated based on fructose toleration and acid and diacetyl production while the probiotic properties of the isolates were determined by examining pH, pepsin, pancreatin resistance, antimicrobial susceptibility, and antimicrobial activity. Anti-QS activities were also evaluated with the
Chromobacterium violaceum
biosensor strain. Two FLAB members were isolated and identified by the 16S rRNA analysis as
Fructobacillus tropaeoli
and
Apilactobacillus kunkeei
, which were found to be tolerant to high fructose, low pH, pepsin, pancreatin, and bile salt environments. Both isolates showed anti-QS activity against the
C. violaceum
biosensor strain and no diacetyl production. The daily supernatants of the isolates inhibited the growth of
Enterococcus faecalis
ATCC 29212 among the selected pathogens. The isolates were found resistant to kanamycin, streptomycin, erythromycin, and clindamycin. In the evaluation of the probiotic potential of these species, the negative effect of antibiotics and other chemicals to which honeybees are directly or indirectly exposed draws attention within the scope of the “One Health” approach.
Graphical abstract